ppt 8 Flashcards

1
Q

What do you use enzymes for, in antibody identification?

A

to help separate the specificities and allow for identification

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2
Q

What antibodies are neutrallized with saliva, plasma, or serum?

A

Lewis Antibodies

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3
Q

How is adsorption used in antibody identification?

A

add a known antigen to the serum, centrifuge and remove antigen/antibody complex, test adsorbed serum for antibodies

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4
Q

What does alloadsorption remove?

A

autoantibodies and alloantibodies

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5
Q

What does autoadsorption do?

A

removes autoantibodies (cold and warm)

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6
Q

What is the procedure for autoadsorption?

A
  1. Incubate cells w/ patient plasma/serum for 1 hr
    1a. Cold autoantibodies at 4C
    1b.. Warm autoantibodies at 37C
  2. If agglutination is present, incubate plasma/serum with new aliquot of autologous RBCs
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7
Q

What is DAT used to detect…

A

in vivo sensitization of RBCs

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8
Q

What is the procedure for DAT antibody identification?

A
  1. RBCs are washed 3x
  2. AHG is added
    2a. Polyspecific AHG, then monospecific after
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9
Q

What is done after IgG are detected by DAT?

A

Eluate (separate Ab from RBC, then use eluate panel to identify the Ab)

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10
Q

What is the Eluate procedure?

A
  1. Heat RBCs at 45C
  2. Acid elution w/ glycine acid solution at 3 pH
  3. Organic acids
  4. Wash
  5. Test Eluate and Last wash supernatant (control)
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11
Q

Titer level is the _______ of the greatest dilution

A

reciprical

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12
Q

Why is initial titration frozen?

A

to test against new specimens as a variability control
(see if technician is doing shit different [ wants similar techniques everytime])

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13
Q

What is titration used for?

A

monitoring patients for transplants and potential HDFN

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14
Q

What do you phenotypically match for with sickle cell and thalassemic patients?

A

Rh and K antigens

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