Quiz 5 Flashcards

You may prefer our related Brainscape-certified flashcards:
1
Q

What is the purpose of southern blot experiment?

A

To study the presence of one gene in multiple species

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
2
Q

Steps of southern blot

A
  • collect in low DNA in different species in different wells of agarose gel
    -Electrophoresis
    -Transfer DNA to nitro cellulose paper
    -Add nuclei acid probe
  • Label fluorescent radioactive
  • chemiluminescent
  • look at the bands
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
3
Q

What is the point of northern blot?

A

For the expression of one gene for different cells of the same species or different species

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
4
Q

Steps for northern Blot

A
  • collect and load RNA in different species and different wells of agarose gel
  • run electrophoresis
  • Blood transfer DNA to nitro cellulose paper
  • add nuclei acid probe
  • if you see bands on the block, gene expression is expressed
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
5
Q

What is rRNA?

A

Ribosomal RNA combined with ribosomal proteins will give you ribosomes

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
6
Q

How many genes are in rRNA processing

A

Two genes: 45s pre rRNA & 5s pre rRNA

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
7
Q

Where does DNA to rRNA occur?

A

Nucleolous

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
8
Q

Steps to rRNA processing

A

-transcription will occur first
-Then ribosomal proteins bind 45 pre-rRNA
-Chemical modifications to pre-RRNA
-Add methyl to basis and sugars to nucleotides and change urine
-Cleve Pre rRNA into 18s, 5.8s, 28s
-generate pre-ribosomal particles (small subunit &large subunit)
- pre-ribosomal particles, exit nucleus, and mature in the cytoplasm

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
9
Q

What is the smallest sub unit made of

A

18 S and proteins

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
10
Q

What is the large Saub unit unit made of

A

5.8 S, 28S, 5S, and proteins

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
11
Q

The large subunits has how many proteins

A

49

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
12
Q

The smallest unit has how many proteins

A

33

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
13
Q

What are the names of the sites in the large Subunit

A

A site P site E site

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
14
Q

What is the A site stand for and what happens there?

A

Aminoacyl site: second tRNA with amino acids and all subsequent tRNA with amino acids will enter

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
15
Q

What does the p site stand for and what happens there?

A

Peptidyl site: site for peptide bond formation

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
16
Q

What does e site stand for and what happens there?

A

Exit site: tRNA W/o amino acid exit here

17
Q

What is tRNA?

A

Transfer RNA

18
Q

tRNA processing steps

A

-generate two prime structure with three hairpins
-Cleve five prime to three prime ends
-add CCA to three prime end of tRNA
-Modify bases
-TNA charging: flip TRNA to be complementary to mRNA, look for codon (mRNA) five prime to three prime, then add sequence of tRNA anticodon.