Brainscape's Knowledge GenomeTM

Browse over 1 million classes created by top students, professors, publishers, and experts.


See full index

Cell Biology > Quiz 3i > Flashcards

Quiz 3i Flashcards

You may prefer our related Brainscape-certified flashcards:
AP® Biology flashcards Biology 101 flashcards
1
Q

What is transcription

A

DNA to RNA

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
2
Q

Where does transcriptions start in the prokaryote?

A

Prescriptions in the cytoplasm

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
3
Q

What factors are in RNA polymerase?

A

Alpha, beta omega, sigma

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
4
Q

What are the steps of transcription?

A

-find promoter sequence
-Label non-template strand
-Sigma with hollow enzyme will bind to promoter
-Count from -10 to plus one and then you arrived at your transcription start site
-Switch to template strand
-Transcribe RNA five, prime to three prime

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
5
Q

What happens in elongation?

A
  • Sigma released, transcription continues using core RNA polymerase
    -Unwound approximately 15 nucleotides have transcription rewind and secrets has been transcribed
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
6
Q

What is termination?

A

Row dependent termination and row independent termination

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
7
Q

How do you terminate transcription?

A

Form a hairpin, which is inverted complementary sequences that forces termination by releasing the core RNA polymerase from DNA and RNA

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
8
Q

Steps of PCR

A
  • heat to the nature 92
    -Chill to anneal 50-60
    -warm to Elongate 72
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
9
Q

What is the purpose of PCR?

A

Create many copies of a region or sequence of DNA

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
10
Q

What is the point of DNA foot printing

A

To determine where protein binds on a sequence

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
11
Q

Steps of DNA foot printing

A

-start with PCR to make copies of regions of DNA
-Label one end of the DNA sequence radioactive and separate DNA into two tubes
- Add a protein that might buy to the DNA in into one and no protein in the other
- Protein will bind to the DNA sequence and protect the DNA from digestion
- DNA into Joe and run electrophoresis
-Shows where the protein was bound

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
12
Q

What is negative inducible

A

It is a repressor protein that’s currently off, but can be turned on, and the repressor is active and bound to the operator, preventing transcription in order to start transcription use a substrate to bind an inactive repressor, and it will unbind from the operator

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
13
Q

What is negative repressible

A

Repressor protein that’s currently on and can be turned off repressor is inactive and bound to the operators, and to stop transcription activate repressor using a product of the pathway

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
14
Q

What’s positive inducible

A

An activator with transcription off and active activator that’s not bound to the operator, and to turn on transcription activate, the activator using the substrate will not bind to the operator

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
15
Q

What is Positive repressible

A

An activator that’s currently on and can be turned off it’s an active activator that’s bound to the operator to turn transcription off and activate the activator using the product to stop the process. This is a form of negative feedback.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
16
Q

What is the nuclear envelope?

A

A barrier between the cytoplasm, and the inside of the nucleus, including DNA

17
Q

What’s included in the nuclear envelope structure?

A

Membrane, nuclear lamina, nucleolus, nuclear poor complex

18
Q

What are the two membranes in the nuclear envelope?

A

Outer and inner nuclear membrane

19
Q

Outer nuclear membrane

A

Has a phospholipid violator, continuous with ER membrane. There can be proteins in membrane, and it can connect to ribosomes and cytoskeleton in the cytoplasm.

20
Q

Inner nuclear membrane

A

Phospholipid by layer has proteins in the membrane, such as emerin. Nuclear membrane will connect to lamin to the lamin B receptor & DNA/ chromatin

21
Q

Nuclear lamina

A

The mesh work or network of lemon proteins, that form intermediate filaments, which maintain the structure of the nucleus

22
Q

The nuclear poor comp

A

Made of proteins called nucleoporins go through the NPC into the nucleus, including erk

23
Q

Nucleolus

A

Site for ribosome generation

24
Q

What does RNA polymerase one involve

A

rRNA

25
Q

What does RNA polymerase two involve

A

mRNA

26
Q

What does RNA polymerase three involve

A

tRNA

27
Q

What is the promoter during a eukaryote transcription?

A

-25 TATAAA

28
Q

Is there an operon in eukaryotic txn?

A

No operon; single genes

29
Q

Steps for eukaryotic transcription

A
  • start with TFIID
  • TFIIA & TFIIB bind to TFIID & DNA
  • TIIF & RNA POL II binds to TFIID,A,B&DNA
  • TFIIE&TFIIH Binds to TFIID,A,B,F& RNA POLII
    -TFIIH acts as helicase that will unwind DNA @1+
  • TFIIH acts as delicase that will phosphorite
30
Q

What is the basil transcription machine?

A

TFIID,A,B,F,E,H& RNA POL II

Cell Biology (10 decks)

Brainscape helps you reach your goals faster, through stronger study habits.
© 2024 Bold Learning Solutions. Terms and Conditions