Quiz 1, WS 1 Lecture 1 and 2

Question 1

red serum/plasma means the sample is what

Answer 1

hemolyzed

Question 2

white serum/plasma means that the sample is what

Answer 2

lipemic

Question 3

yellow serum/plasma means that the sample is what

Answer 3

icteric

Question 4

straw colored/clear serum/plasma means that the sample is what

Answer 4

normal

Question 5

What results from clotting proteins (fibrin) are removed during clotting

Answer 5

serum

Question 6

Results when the fluid portion contains proteins (fibrin) in soluble form

Answer 6

plasma

Question 7

What is used to calibrate the refractometer before and after use?

Answer 7

distilled water

Question 8

red top tube is used for what sample

Answer 8

serum

Question 9

tiger top tube is used for what sample

Answer 9

serum

Question 10

purple top tube is used for what sample

Answer 10

whole blood (plasma)

Question 11

green top tube is used for what sample

Answer 11

plasma

Question 12

blue top tube is used for what sample

Answer 12

plasma

Question 13

additive to red top tube

Answer 13

none - no anticoagulant or separator gel

Question 14

additive to tiger top tube

Answer 14

serum separator (Gel Separator or Clot Activator)

Question 15

additive to purple top tube

Answer 15

EDTA

Question 16

additive to green top tube

Answer 16

heparin

Question 17

additive to blue top tube

Answer 17

sodium citrate

Question 18

when do you centrifuge the red top tube

Answer 18

after the clot has formed

Question 19

when do you centrifuge tiger top tubes

Answer 19

after the clot has formed

Question 20

when do you centrifuge purple top tubes

Answer 20

do not centrifuge

Question 21

when do you centrifuge green top tubes

Answer 21

do not centrifuge

Question 22

when do you centrifuge blue top tubes

Answer 22

ASAP

Question 23

what color tube top would you use for chemistry, serology, most endocrine and chemistry tests

Answer 23

red top or tiger top

Question 24

what color tube top would you use for chemistry tests but not drug concentrations

Answer 24

tiger top

Question 25

what color tube top would you use for running a complete blood count (CBC), hematocrit/packed cell volume, reticulocyte count (immature erythrocytes), or any counts of the aforementioned cells

Answer 25

purple top

Question 26

what color tube top would you use for Knott's heartworm and whole blood toxicology testing of many agents including selenium, lead, and drug screens (blood chemistries)

Answer 26

green top

Question 27

what color tube top would you use for coagulation tests

Answer 27

blue top

Question 28

what is EDTA

Answer 28

anticoagulant

Question 29

what is the definition of clinical pathology

Answer 29

Diagnosing, treating and preventing disease by analyzing bodily specimens and bacteria.

Question 30

what is the definition of hemolysis

Answer 30

Destruction of red blood cells.

Question 31

what is the definition of lipemia

Answer 31

White plasma or serum, due to the sample having a high concentration of lipids.

Question 32

what is the definition of icterus

Answer 32

yellow or jaundice

Question 33

_______ is the _______ portion of blood that has had the ________ proteins removed, a _____ will form.

Answer 33

Serum liquid clotting clot

Question 34

______ is the _______ portion of whole blood that will not let a ______ form.

Answer 34

Plasma liquid clot

Question 35

What do we need to do if a clot forms in a purple top tube? Give an example why this can happen.

Answer 35

Redraw the sample and put the new sample in a new tube. This can happen if the tube wasn’t inverted several times to mix the sample with the anticoagulant.

Question 36

Why is it important not to under fill a treated blood tube?

Answer 36

You want the ratio of blood to chemical in the tube to be correct. Using too much blood will allow a clot to form and using too little blood will dilute the sample (because there is too much anticoagulant).

Question 37

What is the preferred anticoagulant for CBCs?

Answer 37

EDTA

Question 38

Why is it important to separate the RBCs from the plasma or serum promptly?

Answer 38

It can alter the test results if not separated right away.

Question 39

Around how much time does it take for a clot to form completely in a red top tube?

Answer 39

15 to 20 mins

Question 40

name and manufacture of our hematology analyzer

Answer 40

Abaxis HM5

Question 41

name and manufacture of our chemistry analyzer

Answer 41

Abaxis VetScan

Question 42

when nothing was added to the blood it will clot as it sits, using up the clotting factors from the liquid. What is this liquid called?

Answer 42

serum

Question 43

Answer 43

normal blood plasma/serum

Question 44

________ serum

Answer 44

hemolyzed serum

Question 45

Answer 45

lipemic serum/plasma

Question 46

Answer 46

icteric serum

Question 47

which serum sample is normal

Answer 47

far right

Question 48

which serum sample is lipemic

Answer 48

middle right

Question 49

Which serum sample is hemolyzed?

Answer 49

far left

Question 50

which serum sample is icteric

Answer 50

middle left

Question 51

what is the correct order to fill blood tubes

Answer 51

Blue - Sodium Citrate Red - No additive Tiger Top - Gel separator and clot activator Green - Heparin Purple - EDTA Gray - potassium oxalate or sodium fluoride

Question 52

why is it important to fill blood tubes in the correct order?

Answer 52

to avoid cross-contamination of the sample by additives found in different collection tubes

Question 53

what is the PCV (packed cell volume) of a well hydrated animal

Answer 53

50%

Question 54

what is the percent ratio of cells: fluid in a sample from a well hydrated animal

Answer 54

50% cells:50% fluid

Question 55

what is the mL ratio of blood: fluid in a sample from a well hydrated animal

Answer 55

10 mL blood = 5 mL fluid

Question 56

what is the PCV (packed cell volume) of a dehydrated hydrated animal

Answer 56

70%

Question 56

what is the percent ratio of cells: fluid in a sample from a dehydrated animal

Answer 56

70% cells:30% fluid

Question 57

what is the mL ratio of blood: fluid in a sample from a dehydrated animal

Answer 57

10 mL blood = 3 mL fluid

Question 58

Answer 58

normal blood sample

Question 59

What kind of blood sample

Answer 59

anemic blood sample

Question 60

Answer 60

polycythemia blood sample

Question 61

how closely results agree with the true quantitative value of the constituent.

Answer 61

accuracy

Question 62

the magnitude of random errors and the reproducibility of measurements.

Answer 62

precision

Question 63

the ability of a method to be accurate and precise. Factors: test selection, test conditions, sample quality, technician skill, electrical surges, and equipment maintenance.

Answer 63

reliability

Question 64

clerical errors, biologic variables

Answer 64

preanalytical errors

Question 65

instrument errors

Answer 65

analytical errors

Question 66

Errors in data entry/ record keeping, or interpretive errors.

Answer 66

postanalytical errors

Question 67

four things that all samples should be labeled with

Answer 67

owner's last name patient name/ID number species date

Question 68

preferred collection tube for CBCs in mammals and most non mammals

Answer 68

purple top tubes

Question 69

-penia

Answer 69

decreased number of cells

Question 70

-philia

Answer 70

increase number of cells