QUALITATIVE AND SEMIQUANTITATIVE Flashcards
measure the presence or absence of a substance or evaluate cellular characteristics such as morphology.
QUALITATIVE EXAMINATIONS
those that are integrated into the design of a test system such as a kit device.
BUILT IN CONTROLS
those that are integrated into the design of a test system such as kit device.
INTERNAL CONTROLS
made to mimic patient samples and they are tested with the patient samples to evaluate the examination component.
they assess the integrity of the entire system, the suitability of the physical testing environment (temperature, humidity, level workspace), and whether the person conducting the test performs it correctly.
TRADITIONAL CONTROLS
organizations offer reference strains
- AMERICAN TYPE CULTURE COLLECTION (ATCC)
- NATIONAL COLLECTION OF TYPE CULTURE (NCTC)
- PASTEUR INSTITUTE COLLECTION (CIP)
fecal parasites
- ACRIDINE ORANGE
- TRICHOME
- IRON-HAEMATOXYLIN
malaria
GIEMSA STAIN
bacteria and yeast
GRAM STAIN
mycobacterium tuberculosis
ACID FAST STAIN
cyst and egg in fecal samples
IODINE SOLUTIONS
fungal elements
POTASSIUM HYDROXIDE
haematology
WRIGHT STAIN
quality control of microbiological media
- STERILITY - incubate overnight before use
- APPEARANCE - check for turbidity, dryness, evenness of layers, abnormal color
- pH
- ABILITY TO SUPPORT GROWTH - using stock organisms
- ABILITY TO YIELD THE APPROPRIATE BIOCHEMICAL RESULTS
Use of control organisms for verification
Escherichia coli (ATCC 25922): MacConkey or eosin methylene blue (EMB)
Staphylococcus aureus (ATCC 25923): blood agar, mannitol salt and some antimicrobial susceptibility tests
Neisseria gonorrhoeae (ATCC 49226): chocolate agar and Thayer-Martin agar
