Purine, Pyrimidine and Nucleotide Metabolism: Part 1 & 2 Flashcards
what is the cellular location of CPS1
Mitochondria
what is the cellular location of CPS2
Cytosol
which pathway is CPS1 involved
urea cycle
Which pathway is CPS2 involved
Pyrimidine synthesis
what is the source of nitrogen in CPS1
Ammonia
what is the source of nitrogen in CPS2
gamma-amide group of glutamine
what are the regulators of CPS1
activator: N-acetyl-glutamate
what are the regulators of CPS2
activator: ATP
Inhibitor: UTP
______ is the final breakdown product of purines
Uric acid
The degradation of Cytosine leads to ______
Malonyl-CoA
The degradation of Uracil leads to _______
Acetyl-CoA.
The degradation of thymine leads to _______
Succinyl-CoA
what is the enzyme that is lacking in Severe combined immunodeficiency syndrome (SCID)
adenosine deaminase (ADA).
Gout is Caused by _______
overproduction of purine nucleotides via the de novo pathway.As these excess purines are degraded, it leads to uric acid.
Lesch-Nyhan syndrome (LNS) is phenotypically characterized by ______
self-mutilating behaviors such as lip and finger biting and/or head banging
The symptoms of LNS usually appear between the ages of _________.
3 and 6 months
Frequently the first symptom of LNS is ________
the presence of orange-colored crystal-like deposits (orange sand) in the diapers of affected infants
which drugs Block the thymidate synthase/folate metabolism cycle
fluorouracil and methotrexate
A ______ a base combined with a pentose sugar.
If the sugar is phosphorylated, then the molecule is called a ________
Nucleoside,
Nucleotide
The key regulated step of purine de novo synthesis is at the start, when PRPP (contains the ribose sugar) and glutamine are used by ________ to add the first nitrogen to the PRPP
glutamine phosphoribosyl pyrophosphate amidotransferase
The reduction reaction that converts ribose to deoxyribose is catalyzed by an enzyme called
ribonucleotide reductase.
Once UDP is converted to dUDP, it can then be dephosphorylated to make dUMP, which is then converted to dTMP by _________
thymidylate synthase
What is the MOA of 6-mercaptopurine
inhibits AMP synthesis (anti cancer)
What is the MOA of Azidothymidine (AZT):
inhibits viral polymerase (anti HIV)
What is the MOA of Cytosine arabinoside (araC):
targets DNA polymerase (anti leukemia)
What is the MOA of Acyclovir (ACV)
targets viral DNA polymerase and reverse transcriptase (anti Herpes simplex virus)
