Purification and Characterization of Proteins

Question 1

Name the methods that separate proteins by size

Answer 1

• Gel filtration
• Dialysis
• Ultrafiltration

Question 2

Name the methods that separate proteins by charge

Answer 2

• Ion-exchange chromatography
• Affinity chromatography
• High performance liquid chromatography (HPLC)

Question 3

Name the method that separates proteins by purity and molecular weight

Answer 3

SDS-PAGE

Question 4

Name the method that determines the primary sequence of protein

Answer 4

Edman Degradation