PUGAD BIOCHEM Flashcards
Has a polar, uncharged R group
Cysteine
Has a nonpolar R group
Valine
Has a nonpolar R group
Alanine
Has a negatively charge R group
Aspartic acid / Aspartate
The naturally occurring tranquilizer of the body is derived from this amino acid
Glutamic acid
Is the form of a molecule that has an equal number of positive and negative charges and thus is electrically neutral
Isoelectric species
Is the amino acid arrangement maintained by peptide bonds
Primary level
Are maintained by hydrogen bonds between at groups and other special bonds such as the disulfide bridge between cysteine
Tertiary and quarternary level
Are the two most commonly seen secondary level structures.
Alpha helix and beta helix
The stability of it arises primarily from hydrogen bonds formed between the oxygen of the peptide bond carbonyl and the hydrogen atom of the peptide bond nitrogen of the fourth residue down the polypeptide chain
Sheets derive much of their stability from hydrogen bonds between the carbonyl oxygen and amide hydrogen of peptide bonds
Alpha helix
The most visible effect of denaturation on proteins
Decreased solubility
Separates compounds based on their chemical properties and by extension their solubility
Paper chromatography
Separates on the basis of size
Gel-infiltration chromatography
Separates on the basis of their net charge
Ion-exchange chromatography
Takes advantage of the high affinity of many proteins for specific chemical groups
Affinity chromatography