PROTEINS 3

Question 1

MISCELLANEOUS PROTEINS:

1. heme protein or oxygen-binding transport protein found in skeletal & cardiac muscles
   - approx ___% of total muscle protein
   - marker for chest pain (____)
   - early detection of _____
   — onset hrs
   — peak level hrs
   — normalize hrs
   - useful marker for monitoring the success or failure of _____
2. pathological extracellular deposit associated w/ group of disorders called amyloidosis
3. complex of 3 proteins that bind to the thin filaments of striated muscle (cardiac & skeletal
   - diagnostic marker for identifying ______
   - levels in blood may elevate after AMI in the absence of _____
   - 3 muscle contraction
   - Troponin related w/ acute myocardial infarction

Answer 1

1. Myoglobin
   - 2%
   - angina
   - acute myocardial infarction
   — 1-3hrs
   — 5-12hrs
   — 18-30hrs
   - reperfusion
2. Amyloid
3. Troponins
   - cardiac injury
   - CK-MB elevations
   - TnT, TnI, TnC
   - Troponin I

Question 2

MEASUREMENT OF PROTEINS

determination of protein nitrogen derived from constituent amino acids
1. nitrogen in protein free filtrate (pff) is converted to ____
- using _____
2. 3 methods

copper binds to peptide bond structure of the protein
1. ____ chromogen
2. ____ + _____ + _____ + _____

Answer 2

Kjeldahl Method
1. ammonia
- sulfuric acid
2. Nesslerization, Berthelot reaction, Titration method

Biuret Method
1. Purple chromogen
2. protein + copper sulfate + sodium hydroxide + rochelle salt

Question 3

MEASUREMENT OF PROTEINS

widely used in research to measure tissue & enzyme proteins
1. _____ & ____ reduce = ______ reagent
2. _____ color
3. detects proteins in concentrations as low as ______

chiefly the result of high electron density of aromatic rings of tyrosine & tryptophan in solution
1. pH
2. best method for

Answer 3

Folin- Ciocalteu
1. tyrosine & tryptophan = phosphotungstic & phosphomolybdic acid reagent
2. Blue color
3. 10-60 ug/mL

Absorption of UV light at 280nm
1. 8
2. CSF

Question 4

MEASUREMENT OF PROTEINS

ability of proteins to bind certain dyes
1. linearity up to
2. used to stain protein bands after
3. method for albumin
- pH
4. give 3 other dyes

often used for measuring urine & CSF protein
1. 2 precipitating agents:
-
-

Answer 4

Dye-Binding Methods
1. 150mg/dL
2. electrophoresis
3. Bromcresol Green Method
- 4.2
4. Amino Black 10B, bromcresol purple, methyl orange

Turbidimetry & Nephelometry
1.
- sulfosalicylic acid (SSA) w/ sodium sulfate
- Trichloroacetic acid

Question 5

MEASUREMENT OF PROTEINS

_____: direction of migration of proteins in electrical field

1. determined by _____ & ______
2. exactly same as serum except it must be concentrated before application
3. support media (3)
4. stains (3)

Answer 5

Protein Electrophoresis

1. surface charge &
   molecular weight of protein
2. Urine protein electrophoresis
   3.
   -cellulose acetate
   -agarose gel
   -starch gel
   4.
   -amido black
   -ponceau S
   -coomassie brilliant blue

Question 6

Protein Electrophoresis

1. protein DONATES protons = (-) charge
   - pH
   - migrate towards
2. protein RECEIVES protons = (+)
   - pH
   - migrate towards
3. causes γ-globulins to migrate toward CATHODE even though
   they are slightly (-) charged
   - at pH ___ = in order of migration

Answer 6

1.
- ALKALINE
- ANODE
2.
- ACIDIC
- CATHODE
3. ELECTROENDOSMOSIS
- 8.6