Glucose Methods

Question 1

CHEMICAL METHODS

Alkaline Copper Reduction

1. total reducing substances (true reducing substances and saccharoids) measured
2. enables measurement of true reducing substances only
   - ___ enables measurement of true reading substance only
3. method which have end product of cuprous-neocuproine complex
   - color of end product

_____: reverse colorimetric method

1. method
2. 3 methods

Answer 1

Alkaline Copper Reduction

1. FOLIN WU
2. NELSON-SOMOGYI
   - BARIUM SULFATE
3. NEUCUPEOINE CAMPBELL & KING METHOD
   - yellow-orange

Alkaline Ferric Reduction

1. Ferricyanide —> Ferrocyanide
2. Johnson, Prussian Blue, Jensen method

Question 2

CONDENSATION METHOD

w/ aromatic amines (reference method
1. method
2. color
3. wavelength

w/ phenol
1. color
2. product

Answer 2

w/ aromatic amines (reference method
1. ortho-toluidine method by Dubowski
2. green
3. 630nm

w/ phenol
1. green
2. anthrone

Question 3

ENZYMATIC METHODS

_____: most specific enzyme reacting w/ only β-D-glucose

1. end product
2. end product after oxidation
3. added to convert alpha to beta
4. falsely decrease (3)
5. falsely increase
6. wavelength

Answer 3

Glucose Oxidase Method

1. gluconic acid + H2O2
2. oxidized chromogen + H2O
3. mutarotase
4. increased levels of uric acid, bilirubin, & ascorbic acid
5. hemolysis = hgb has pseudoperoxidase activity
6. 480-520nm

Question 4

ENZYMATIC METHODS

methods to quantify hydrogen peroxide:

Gochman & Schmitz
1. color of end product (name of dye)
2. wavelength

Trinder
1. color
2. name of dye

Miskieweis
1. product

_____: measures the amount of oxygen consumed in glucose
oxidase method

____: self-monitoring of blood glucose

Answer 4

methods to quantify hydrogen peroxide:

Gochman & Schmitz
1. Purple (indamine dye)
2. 590-660nm

Trinder
1. Red
2. Quinoneimine

Miskieweis
1. colored chromogen

Polarographic Oxygen Electrode
Point of Care device

Question 5

1. more accurate, less interferences than glucose oxidase (reference)
   - false decrease (2)
   - method
   - color
   - wavelength
2. last method
   - coenzyme 1
   - coenzyme 2

Answer 5

1. Hexokinase Method
   - hemolyzed sample and elevated bilirubin levels
   - Keller Method
   - Red
   - 340nm
2. Glucose Dehydrogenase Method
   - NAD
   - NADP