Proteins Flashcards

1
Q

Protein are made up of…

A

carbon, oxyen, nitrogen, hydroen, sulfur, and phosphorus

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2
Q

do animals have a protein req?

A

no, just an amino acid requirement

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3
Q

What structure has biologic activity with true food allergy?

A

tertiary. this structure looks foreign to the bdy and makes an antibdy to it. Protein hydrolysate via enzyme bacteria is used to make the immune system not recognize it.

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4
Q

What are simple proteins

A

fibrous (collagen, elastin and keratin)

globular (enzymes, homones, antibodies)

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5
Q

what are conjugated proteins

A
Glycoproteins (protein with a carbohydrate)
	food allergens
Lipoproteins (protein w a lipid)
	chylomicrons, VLDL, LDL,HDL
Metalloproteins (protein w a metal)
	hemoglobin, cytochromes, caseins
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6
Q

What are some nitrogen containing protein compounds

A

urea and ammonia

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7
Q

how much protein in each area of the body

A

33% in muscle
37% in body fluids
20% in bone and cartilage
10% in skin

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8
Q

what are the functions of proteins

A

structural (collagen, elastin, actin/myosin, keratin)
blood proteins (hemoglobin, transferrin, albumin, globulin)
energy (gluconeogenesis)
other-enzymes, hormones, antibodies

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9
Q

Where are proteins digested ??

A

Dogs and cats: stomach: by pepsin(meats) and HCL
small int.: pancreatic enzymes-trypsin chymotripsin,
and carboxypeptidases
brush border enzymes
4 diff amino aacid carrier systems
Ruminants: Abomasum: HCL

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10
Q

What enzymes break down proteins

A
pepsin (from pepsinogen and HCL and pepsin)
Rennin (from chymosinogen)
Trypsin (from trysinogen)
chymmotrypsin (chymotrypsinogen)
Elastase (from pro-elastse)
Carboxypep A (from procarboxypep. A)
Carboxypep B (from procarboxypep B)
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11
Q

What is Rennin

A

comes from Rennet: is a natural complex ofenzymes produced in any mammalian stomach to digest the mothers milk
another name for rennin is chymosin
coagulates milkcausing it to seperate into curds and whey
used to coagulate milk in the making of cheese

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12
Q

Membrane bound peptidases

A

apical membrane of mucosal cells
hydrolyze peptide products of luminal phase
in the small int. produce free amino acids, di and tripeptides
for absorption

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13
Q

Absorption of free amino acids is

A

sodium dependant
there are co transport systems for aa in neutral, acidic, or basic aa.
sodium amino co transport system for absorption of proline and hydroxyproline.

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14
Q

Absorptino of di and tri peptides are done by

A

transported across apical membranes and are converted to free amino acids

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15
Q

how does ruminant digestion work/? whatisthe fate of a dietary protein or non protein nitrogen

A
  1. Rumen undegradable protein (RUP) escape rumen digestion in the abomasum
  2. microbial fermentation of protein and NPN
  3. fermentation of ruminal nitrogen is converted to bacterial protein in the abomasum
  4. ammonia is converted to urea in the liver and is recycled back into the rumen via saliva or excreted by the kidneys
  5. abomasal protein is made , it is a mixture ofmicrobial protien and RUP
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16
Q

Proteins are broken down into amino acids, then delivered to the liver what does the liver send out?

A
  1. serum proteins
  2. amino acids
  3. tissue (kerratin, collagen, and elastin)
  4. blood cell production
  5. antibodies and hormones
  6. Urea – to make energy or kidneys to make urine
    this is a loss of ammonia
17
Q

cats have a very active ___ cycle

A

urea cycle.
because ammonia is very toxic and must be converted
gluconeogenesis makes lots of ammonia

18
Q

what amino acid is highly essential for cats?

A

argenine

19
Q

What is protein turnover of metabolism

A

A continuous process of releasing amino acids from muscle and putting the back in.
muscle and plasma proteins have long life
nitrogen is lost–but there is a balance bw nitrogen consumed and nitrogen lost

20
Q

What is crude protein

A

Apparent = crude protein - fecal protein

True = crude protein - illial protein (use a tube to sample through)

sulfuric acid + whole food –digest– = nitrogen determination x 16%= crude protein of the diet

21
Q

what affects the amount of protein which must be fed to the patient?

A

quality.

higher quality protein means a lower dietary protein level needed

22
Q

What are the dietary essential amino acids

A

methionine, arginine, tryptophan, threonin, histidine, isoleucine, leucine, lysine, valine,phenylalanine, taurine

23
Q

How are protein requirements calculated?

A

from the amino acid requirement converted to nitrogen requirement n expressed as protein requirement.
this is about 50% of essential aa
and 50 % non essential aa

24
Q

amino acid requirement will tell

A

how much protein.
depends on life stage, essential amino acids and extras for non essential, the exact amino acid req is not knwn for every disease state.

25
Q

Can you have a toxic amount of amino acids

A

no notwith animal and plant protein sources