Proteins Flashcards

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1
Q

What are proteins made up of?

A

Polymers made up of many amino acids

Proteins are also known as polypeptides.

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2
Q

What is a polypeptide?

A

A polymer made up of many amino acids

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3
Q

What are the three groups contained in all amino acids?

A
  • Carboxyl group
  • Amino group
  • Variable (R) group
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4
Q

How many different amino acids are there?

A

20

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5
Q

What do R-groups change in amino acids?

A

The properties of the amino acid

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6
Q

What type of bond joins adjacent amino acids?

A

Peptide bonds

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7
Q

What is a dipeptide?

A

Two amino acids joined together

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8
Q

How is a dipeptide formed?

A

During a condensation reaction forming a peptide bond

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9
Q

What are the four different levels of protein structure?

A
  • Primary
  • Secondary
  • Tertiary
  • Quaternary
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10
Q

What does the primary structure of a protein refer to?

A

The order of amino acids in a polypeptide

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11
Q

What types of structures are included in the secondary structure of proteins?

A
  • α-helix
  • β-pleated sheet
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12
Q

How do secondary structures form?

A

Via hydrogen bonds

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13
Q

What defines the tertiary structure of a protein?

A

The final 3D structure of a polypeptide caused by further folding

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14
Q

What interactions contribute to the tertiary structure?

A
  • Hydrogen bonds (polar groups)
  • Ionic bonds (charged groups)
  • Disulfide bridges (groups containing S)
  • Hydrophobic regions
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15
Q

What is the quaternary structure of a protein?

A

The joining of multiple polypeptides together

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16
Q

What are prosthetic groups?

A

Non-protein groups associated with conjugated proteins

17
Q

What determines a protein’s function?

A

Its tertiary or quaternary structure

18
Q

True or False: A change in a protein’s structure can affect its function.

A

True

19
Q

What genetic mutation causes sickle cell disease?

A

A mutation in the gene for haemoglobin

20
Q

What amino acid change occurs in sickle cell disease?

A

Glutamine is swapped for valine

21
Q

How does the mutation in sickle cell disease affect protein structure?

A

It causes a change in the primary structure, which alters the tertiary structure

22
Q

What is the biochemical test for proteins?

A

Biuret test

23
Q

What is the procedure for the Biuret test?

A

Add NaOH to sample, then add a few drops of dilute CuSO4

24
Q

What color change indicates the presence of proteins in the Biuret test?

A

Blue to purple