Proteins Flashcards

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1
Q

Elements

A
  • C
  • H
  • O
  • N
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2
Q

R group

A
  • could contain other elements
  • sulphur - covalent bonding, strong, enzymes
  • phosphorus
  • iron - RBC
  • molecule variety
  • 20 amino acids in total
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3
Q

Amino acids

A
  • methionine = start codon

- cysteine = disulfide bridge = H-S-S-H very strong

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4
Q

Structure

A
  • amino group = H-N-H
  • R group = R-C-H
  • Carboxyl group = O=C-OH
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5
Q

dipeptide

A
  • condensation reaction

- peptide bond

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6
Q

Primary organisation

A
  • straight chain

- peptide bonds

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7
Q

Secondary organisation

A
  • alpha helix
  • beta pleated sheet
  • peptide bonds
  • disulfide bonds
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8
Q

Tertiary organisation

A
  • peptide bond
  • hydrogen bonds
  • ionic interaction
  • disulfide bridge
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9
Q

quatemary

A
  • more than 1 polypeptide chain
  • peptide bond
  • hydrogen bond
  • ionic interaction
  • disulfide bond
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10
Q

fibrous

A
  • long and narrow
  • structural
  • repetitive amino acid sequence
  • less sensitive to changes in pH, temperature etc
  • collagen, myosin, fibrin
  • insoluble in water
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11
Q

globular

A
  • round / spherical
  • functional
  • irregular AA sequence
  • more sensitive to changes
  • enzymes, haemoglobin
  • soluble in water
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12
Q

buirtet test for protiens

A
  • test solution needs to be alkaline so add few drops of sodium hydroxide
  • ad biuret solution
  • turns blue to purple
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