benedict's test

Question 1

reducing sugars

Answer 1

• add benedict’s reagent (blue) to sample
• heat in a water bath
• positive = green, yellow, orange, brick red

Question 2

Accuracy

Answer 2

• filter solution and weigh precipitate

- remove ppt and use a colourimeters to measure absorbance of remaining benedict’s solution

Question 3

Non reducing sugars

Answer 3

• add dilute hydrochloric acid to break it into monosaccharides
• heat in water bath
• neutralise by adding sodium hydrogen carbonate
• continue as rest of reducing sugar test