Protein Synthesis Flashcards
Transcription
mRNA using DNA as a template
Helicase
uncoils double helix of DNA
mRNA is formed
When RNA nucleotides (RNA polymerase) form base pairs with the exposed DNA
After mRNA is formed
The message from the DNA is transcribed onto the the RNA
Using restriction enzymes
the non coding regions are removed from the copied mRNA
Finally mRNA does what?
Detaches from the strand and moves out into the cytoplasm
Translation
Protein production at a ribosome
Cytoplasm contains what?
tRNA molecules, each carrying a single amino acid
Determined by the codons on mRNA
the tRNA carrying a complementary code(anticodon) bonds to a mRNA
In the ribosome
The codons are processed (two at a time), the amino acids are separated from the tRNA and join up to form a protein
Histones do what
Make the protein shape
Washing up liquid
Disrupts the membrane
Sodium chloride (salt)
Clumps the DNA
Protease
Breaks down the protein in the chromosome
Freezer-cold ethanol
Separate the DNA