Protein Synthesis Flashcards
Explain the flow of genetic info
Transcription -> Pre mRNA -> Capping / PolyA tail / Splicing -> Mature mRNA
How does the cell know when to undergo translation?
mRNA is exported out the nucleus and recognised by the ribosome for translation
Describe the structure of eukaryotic mRNA
5' capped end followed by 5' UTR Coding region in the middle 3' UTR 3' polyAdenylated tract
What are the consequences of mRNA modification errors?
Protein is unable to leave the cell and is degraded
What does the coding region consist of?
Exons
including the initiaton codon
What is the initiation codon?
AUG methionine
How many bases are read at a time by the ribosome?
3 bases / 1 codon
How are the amino acids assembled at the ribosome?
Complementary anitcodon to mRNA codon on tRNA
Amino acids on 3’ end of tRNA
form peptide bonds
What is the role of the 5’ UTR?
Determines rate of protein synthesis
What is the significance of the 3’ UTR ?
Affects stability
Explain what it means when we describe the genetic code as universal?
More than one codon can code for the same amino acid
Describe the consequences of a transcription / translation error
Unable to fold protein correctly, structure changes = non functional
What are ribosomes composed of?
rRNA and ribonucleoprotein complexes (protein)
What are the 2 types of ribosome?
- ribosomes free in cytosol
- ribosomes bound to ER
Where does protein synthesis occur?
At a cleft between a large and small ribosomal subunit