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FLC > Cell Cycle > Flashcards

Cell Cycle Flashcards

1
Q

What is the cell cycle?

A

Fundamental mechanism of all living things to reproduce and pass down genetic material

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2
Q

How is purity of genetic material maintained?

A

Through high regulation mechanisms

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3
Q

What are unicellular organisms?

A

Bacteria & yeast

Each cell cycle gives rise to 2 new organelles

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4
Q

How do multicellular organisms undergo cell division?

A

A single fertilised egg (zygote) undergoes many rounds of cell division to produce a new fully grown organism

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5
Q

What changes does a cell undergo during the cell cycle?

A
  • Cell contents (DNA, organelles, cytoplasm) duplicated

- Divide into new progeny (daughter) cells

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6
Q

Apart from fertilisation, when else do multicellular organisms cells replicate?

A

Continuously replicate any dying cells during their lifetime

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7
Q

What phases does the cell cycle consist of?

A

G1 -> S phase -> G2 -> M phase

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8
Q

What is the G0 phase?

A

Cellular state outside of the replicative cycle

- can be a temporary resting period or permanent

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9
Q

What happens to cells in G1 phase?

A

DNA content is diploid (2n)

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10
Q

Which phases of the cell cycle are part of interphase?

A

G1, S, and G2

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11
Q

What occurs in the G2 phase of the cell cycle?

A

Cell growth and protein synthesis

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12
Q

What occurs during S phase of the cell cycle?

A

DNA synthesis leads to duplication of chromosomes

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13
Q

What happens in M phase?

A

Cell division occurs - meiosis/mitosis

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14
Q

How is hepatocytes replication regulated?

A

Hepatocytes are maintained in G0 phase unless stimulated to divide

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15
Q

How does the DNA content of cells change during the cell cycle?

A

DNA content doubles (4N) to diploid chromosome number in M phase

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16
Q

Is it possible to re enter the cell cycle?

A

No re entry into the cell cycle is not possible

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17
Q

What stages does the M phase consist of?

A
  1. Mitosis

2. Cytokinesis

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18
Q

What is a contractile ring?

A

Cytoskeletal structure composed of actin and myosin tubules

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19
Q

Give examples of cells constantly in the replicative cell cycle

A
  • epithelial cells (gut)

- hematopoetic cells (bone marrow)

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20
Q

List the main features of Mitosis

A
  • 2 daughter cells
  • Diploid DNA
  • 1 Cell division
  • No recombination of homologus chromosomes
  • Daughter cells are clones of the parent
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21
Q

How is genetic diversity introduced during meiosis?

A

Recombination occurs between homologous chromosomes

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22
Q

How is the cell cycle regulated?

A

Via the G1, G2 and M checkpoints

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23
Q

Describe the structure of centrosomes

A

Consists of a pair of centrioles surrounded by a pericentriolar matrix

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24
Q

What occurs during Meiosis II 2nd cell division?

A

Sister chromatids line up at spindle

Separated to opposite spindle fibres

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25
Q

How do the concentrations of CDK & cyclin vary throughout the cell cycle?

A

CDK levels remain stable

Cyclin levels vary

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26
Q

Explain how cytokinesis occurs

A
  1. Contractile ring accumulates beneath plasma
    membrane at mitotic spindle poles
  2. Ring contracts forming an indentation
  3. Cleavage splits cell in 2
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27
Q

Explain in detail what occurs at the G1 checkpoint in the cell cycle

A

If environment contains enough growth factors
1. Mitogenic signal induces Cyclin D
2. Cyclin D binds to CDK4 forming a complex
3. This complex phosphorylates PRB
4. PRB binds to E2F which is then sequestered
(deactivated)
5. CDK4 phosphorylates PRB, freeing E2F
6. E2F can now promote transcription for target genes
(cyclin A and B) into S phase

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28
Q

Outline the centrosome cycle that aids cell cycle regulation

A
  1. Duplicate during interphase

2. Migrate to opposite poles in preparation of M phase

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29
Q

What factors of the cell cycle have to be strictly regulated?

A

Entry into cell cycle
Each phase occur once per cycle
Correct order of phases
Non overlapping of phases

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30
Q

Give an example of cells permanently in the G0 phase

A

Neurons

31
Q

Explain what happens in the 1st cell division during meiosis I

A

Homologous chromosomes line up at equator on spindles

Pulled apart to opposite poles

32
Q

Which cyclins and CDKs are regulating the cell cycle at each phase?

A
  1. Cyclin D and CDK4 are active in G1 phase
  2. Cyclin E and CDK2 are active during end of G1 and start of S phase
    • smooths out the transition between stages
  3. Cyclin A and CDK2 ensure DNA Is replicated correctly in S phase
  4. Cyclin B and CDK1 are active through G2 and M phase
33
Q

What is Cytokinesis?

A

The division of the cytoplasm and organelles

34
Q

What are the Sex chromosome nondisjunctions?

A

XO - Turner’s syndrome
XXX - Triple X syndrome
XXY - Klinefelters Syndrome

35
Q

What occurs during Mitosis?

A

The cell nucleus divides

36
Q

What occurs in the G0 phase?

A

Cells leave the replication cycle and stop dividing either temporarily or permanently

37
Q

What are the 2 cell cycle regulators?

A
  1. Cyclin dependent Kinases (CDK)

2. Cyclins

38
Q

How do CDKs and Cyclin regulate the cell cycle in humans?

A

CDK binds to cyclin to become activate
The complex can phosphorylate target proteins
Active CDK triggers next phase in the cell cycle
Cyclin degradation terminates CDK activity

39
Q

What are centrosomes?

A

Cell organelles known as Microtubule organising centres in somatic animal cells

40
Q

How are mutations prevented at G1 phase?

A

DNA damage repair also occurs at G1 checkpoint and are dealt with before entry into S phase

41
Q

During which phases do the 2 rounds of cell division in meiosis occur?

A

Cell divisions occur in Metaphase I and Metaphase II

during meiosis I and II

42
Q

What does the M phase checkpoint detect?

A

Checks if all chromosomes are aligned properly

43
Q

What is meant by autosomal trisomy?

A

A chromosome other than the sex X and Y is present in 3 copies

44
Q

What are mitotic spindles?

A

Bipolar array of microtubules

Assembled during prophase from the centrosomes at each pole

45
Q

What is the consequence of nondisjunction?

A 
Autosomal nondisjunction (trisomy) is usually fatal (with 3 exceptions)
Results in daughter cells with abnormal chromosome numbers (aneuploidy)
46
Q

What are the products of Meiosis?

A

Gametes (sperm and egg cells)

47
Q

How do spindles attach to chromosomes?

A

Via the kinetochore

48
Q

What is P53?

A

A tumour gene aka TP53 (tumour protein)

49
Q

Explain what is meant by chromatids?

A

2 chromosomes linked via the centromere that have been duplicated

50
Q

Under normal conditions what is the fate of P53?

A

Normally P53 is degraded quickly as it is unstable

- maintained at low levels

51
Q

What are the distinguishing features of meiosis?

A
  • 4 daughter cells
  • Haploid DNA
  • 2 Cell divisions
  • Homologous recombination occurs (independent
    segregation etc.)
52
Q

What is the role of Cyclins?

A

CDK regulators

- different cyclins produced at each phase of cell cycle

53
Q

What is the centomere?

A

Part of the chromosome connected to spindle fibre

54
Q

When does the G2 checkpoint occur in the cell cycle?

A

Before the M phase

55
Q

What is Nondisjunction?

A

Failure of homologous chromosomes or sister chromatids to separate from one another during
meiotic divisions I or II or during mitosis

56
Q

Which checks are carried out at the G1 checkpoint?

A

Check if

  • extracellular environment is favourable
  • growth factors available
  • DNA damaged
57
Q

What happens when P53 gene is phosphorylated?

A

Phosphorylated P53 is not degraded

Active P53 promotes transcription of genes that induce cell cycle arrest

58
Q

Define kinetochre

A

Protein structure formed at chromatids, to which spindle fibres attach to pull chromatids apart

59
Q

Explain what CDKs are

A

Cyclin dependent Kinases are enzymes that phosphorylate target proteins
- they become active when bound to a corresponding
protein

60
Q

What are the 3 Autosomal trisomy’s?

A

Downs Syndrome - trisomy 21
Edwards Syndrome - Trisomy 18
Patau Syndrome - trisomy 13

61
Q

What are 3 different types of spindle microtubules?

A
  • Astral microtubules
  • Kinetochore microtubules
  • Interpolar microtubules
62
Q

Outline the 5 stages of Mitosis and what occurs during each step

A
  1. Prophase
    • Chromosomes condense
    • Centorosomes move to opposite poles
    • Mitotic spindles form
  2. Prometaphase
    • Nuclear envelope disappears
    • Chromosomes attach to mitotic spindle
  3. Metaphase
    • Centrosomes at opposite poles
    • Chromosomes line up at the equator
  4. Anaphase
    • Sister chromatids pulled to opposite poles by spindle fibres at their centromeres
    • New daughter chromosomes at opposite poles
  5. Telophase
    • Chromosomes arrive at spindle fibre
    • Chromosomes decondense
    • Nuclear envelope reforms
63
Q

What are the two families of CDKI?

A

INK4 - Inhibitors of Kinase 4

CIP/KIP - CDK inhibitory protein / Kinase inhibitory protein

64
Q

What causes neoplasia?

A

Misregulation of the cell cycle causes cancer

65
Q

How does P53 induce cell cycle arrest?

A
  1. P53 binds to promoter region of P21 gene
  2. Stimulates P21 expression
  3. P21 binds and nhibits G1-CDK and S-CDK complexes
66
Q

What is the consequence of DNA not repairing in G1?

A

The cells undergo apoptosis

67
Q

What does the G2 checkpoint look out for?

A

Checks if DNA replication occured correctly and for DNA damage

68
Q

Define Neoplasia

A

The presence or formation of new, abnormal growth of tissue

69
Q

What is the role of INK4?

A

Specifically inhibit G1 CDKs (e.g. CDK4)

70
Q

What is the purpose of cell cycle arrests?

A

Cell arrests in G1 allow time for DNA repair

71
Q

What are Cyclin dependent kinase inhibitors?

A

Proteins that inhibit CDK

72
Q

Where in the cell cycle does G1 checkpoint occur?

A

Just before the S phase

73
Q

How can misregulation of the cell cycle occur?

A
  • Cells escape normal cycle checkpoints; uncontrolled
    progression through cell cycle
  • Genes regulating the cycle can be mutated
74
Q

What is the role of CIP/KIP?

A

Inhibit all other CDK-Cyclin complexes (late G1,G2 and M phase)
gradually sequestered by G1 CDKs - allowing the activation of later CDKs

FLC (34 decks)

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