protein synthesis Flashcards

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1
Q

what are the two processes involved in protein synthesis

A

transcription and translation

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2
Q

what are the problems when making proteins

A

DNA located in nucleus but proteins made in cytoplasm.
-DNA too large to leave nucleus so a copy of a section is made called mRNA.

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3
Q

describe the process of transcription.

A

DNA unwinds with the enzyme DNA helicase and hydrogen bonds break between bonds. The mRNA is built up by complementary base pairings using a DNA template and RNA polymerase joins nucleotides together to complementary base pairing using phosphodiester bonds

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4
Q

what is a codon

A

in mRNA a set of 3 bases.

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5
Q

what are the post transcriptional stages

A

enzymes splice introns as unwanted

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6
Q

what is the problems if introns were not spliced

A

code for a different protein and don’t code for amino acids.

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7
Q

describe what happens during translation

A

polypeptide chain is formed ribosomes assembled in nucleolus from rRNA and protein. Has two subunits with a groove for mRNA.

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8
Q

What is tRNA

A

amino aicds attach to tRNA-each tRNA is specific for one amino acid and has a sequence of 3 bases called the anticodon, complementary to codon on mRNA.

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9
Q

what is amino acid activation

A

a specific tRNA molecules are attached to specific amino acids in the presence of ATP and enzymes.

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10
Q

what are pruines

A

-arebigger and have a two ring strcutre-adenine and guanine.

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11
Q

what is the sugar-phosphate backbone

A

where nucleotides join together to form a sugar to phosphate to sugar chain and are joined by strong covalent phosphodiester bonds.

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12
Q

what are exons

A

expressed DNA that codes for amino acids

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13
Q

what is the structure of tRNA

A

at one end there is a set of 3 unpaired bases called anticodon.
At the other end there is an amino acid binding site which binds to a specific amino acid dependant on anti codon sequence.

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14
Q

what are fibrous proteins

A

long and thin and shape makes them insoluble in water -collagen is a fibrous protein.
-secondary protein structure.
-strength/structural features.

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15
Q

what is a globular protein

A

spherical, unstable and soluble- have metabolic function such as enzymes.

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