protein synthesis Flashcards
what are the two processes involved in protein synthesis
transcription and translation
what are the problems when making proteins
DNA located in nucleus but proteins made in cytoplasm.
-DNA too large to leave nucleus so a copy of a section is made called mRNA.
describe the process of transcription.
DNA unwinds with the enzyme DNA helicase and hydrogen bonds break between bonds. The mRNA is built up by complementary base pairings using a DNA template and RNA polymerase joins nucleotides together to complementary base pairing using phosphodiester bonds
what is a codon
in mRNA a set of 3 bases.
what are the post transcriptional stages
enzymes splice introns as unwanted
what is the problems if introns were not spliced
code for a different protein and don’t code for amino acids.
describe what happens during translation
polypeptide chain is formed ribosomes assembled in nucleolus from rRNA and protein. Has two subunits with a groove for mRNA.
What is tRNA
amino aicds attach to tRNA-each tRNA is specific for one amino acid and has a sequence of 3 bases called the anticodon, complementary to codon on mRNA.
what is amino acid activation
a specific tRNA molecules are attached to specific amino acids in the presence of ATP and enzymes.
what are pruines
-arebigger and have a two ring strcutre-adenine and guanine.
what is the sugar-phosphate backbone
where nucleotides join together to form a sugar to phosphate to sugar chain and are joined by strong covalent phosphodiester bonds.
what are exons
expressed DNA that codes for amino acids
what is the structure of tRNA
at one end there is a set of 3 unpaired bases called anticodon.
At the other end there is an amino acid binding site which binds to a specific amino acid dependant on anti codon sequence.
what are fibrous proteins
long and thin and shape makes them insoluble in water -collagen is a fibrous protein.
-secondary protein structure.
-strength/structural features.
what is a globular protein
spherical, unstable and soluble- have metabolic function such as enzymes.