Protein Regulation Flashcards
What are the methods of protein localisation
Temporal and spatial (when and the quality)
Post transcriptional modifications
Scaffolding complexes (making a larger complex)
Specific localisation (creating a protein for a specific area)
Why would ph need to be controlled
A certain ph would be needed for specific environments and to activate the protein
If not would have bonds break, domains released and the inactivation (no more translation)
What are protein domains
Areas of he protein that would allow the protein to carry out a specific function
For example camodulin (able to form calcium camodulin when smooth muscle contraction occurs)
How would atp be used for the movement of molecules
When atp hydrolysed to ADP and pi would allow the movement
How would ATP allow the opening and closing of the channels
ATP attached closes opens the channel
ATP hydrolysed to ADP and Pi would close the channel
What are the components of the Rho GTPase and what do they do
GAP: converts GTP to GDP (inactivated)
GEF: converts GDP to GTP (activation)
GDI: attached to the Other Rho GTPase molecules and allows them to remain inactive
What would happen if the levels of GEF were low
No converstion of GDP to GTP
The levels of GAP would remain high
Lots of GTP to GDP
Would have inactivation
What would happen if the levels of GAP were low
No GTP to GDP
Large levels of GEF still
Lots of GDP to GTP
Constant activation
What would phosphorylation and what would dephosphorylate
Protein kinase: phosphorylation
Protein phosphatese: dephosphorylates
What amino acids can be phosphorylated
Seronine
Theronine
Tyrosine
What are the two ways that active muscle glycogen phosphorylase can be produced
By AMP addition
By phosphorylation (phosphorylase kinase which would need ATP energy)
What would be needed for phosphorylation
ATP energy
What lysine group would be needed for protein ubiquination
Lysine 48
What lysine group would be needed for DNA repair
Lysine 63
How would a proteasome work
Regulatory region would attach to the protein that would need to be degraded (atp)
Proteasome complex formed
Protein degraded
