Post Translational Modifications, protein Targetting And Collagen Biosynthesis Flashcards
How many helixes are on collagen
3
Every third amino acid would be glycine
Also large amount is Serine and proline
Where are the hydrogen bonds and why would then be there
Within the helix structure
Would allow increased stability in the molecule
What would be needed for hydroxylation
Vitamins c
What does hydroxylation help with
Thermostability
What are the two terminals that’s would be found within the procollagen
N terminal
C terminal
What terminal would have the disulfide bonds
The C terminal
What causes scurvy and what are some of the symptoms
Deficiency of vitamin c or ascortic acid
Weakness, poor healing, gum disease, anaemia
What is osteogenesis imperfecta
The mutation in the COL1A1 or COL1A2 genes
Would have glycine substituted for a bulkier amino acid
Not having the correct folding of the molecule
What is elders-Danlos syndrome
Stretchy skin
Mutation in the collagen type V or deficiency in the Lysyl oxidase enzymes (not allowing the cores bridges to form)
Would effect the extra cellular cleavage, the cross link formation and stability would be effected
What are the steps that would allow the formation of the cross bridges
Lysine residues
Lysyl oxidase and oxygen forming the aldehyde derivatives
Formation of the Aldo’s cross links (thought to be spontaneous)
What cleaves the terminals on pro-collagen to form tropocollagen
Collagen peptidase
What is needed for collagen to enter the endoplasmic reticulum
The signalling function that would soon be cleaved off (signal pepsidase)
What stages would occur I the extracellular
Cleavage of the n and c terminals to for tropocollagen
The formation of the cross bridges
How would preproinsulin go to proinsulin
Cleavage of the signalling function
Formation of the disulfide bonds
How would proinsulin go to insulin
The cleavage of the C-terminal in the Golgi apparatus