protein production Flashcards
exam 1
when does cysteine form disufide bonds?
in an oxidizing environment; second step on making insulin
precursor and final forms of insulin
preproinsulin–> proinsulin–> insulin
some proteins are _________ to the mature form by removal of a _____ _______ peptide and an _____ peptide
Some proteins are proteolyzed to the mature form by removal of a N-terminal peptide and an internal peptide
folding and structure of ___ ____ ______ is complex
rod like collagen
precursor and final forms of collagen
preprocollagen—–> procollagen—> collagen
Collagen contains hydroxylated _______ and ______
prolines and lysines
co factor for hydroxylation
vitamin c
deficiency in vitamin c leads to
scurvy
purple spots on skin
scurvy
The triple-helix of procollagen folds from the _____ terminal to the ______ terminal and is nucleated by _____ ____ formation in the C-terminal propeptide
The triple helix of procollagen folds from the C’end terminal to the N- end terminal and is nucleated by disulfide bond formation in the C-terminal propeptide
upon secretion of procollagen from the cell, it is cleaved by _____ to form ______
Upon secretion of procollagen from the cell, it is cleaved by propeptidases to form tropocollagen
tropocollagen spontaneously assemblies into
immature fribril
The fibril of tropocollagen is strengthened by ________ between ____ residue and ______ residue
The fribril of tropocollagen is strengthened by cross-linking between a lysine residue and an allysine residues
Most of the crosslinking are between _________ molecules in the ______
Most of the crosslinking are betweeen tropocollagen in the fibril
Allysine residue are made by action of ______ _____ acting on a particular _____ and ______ residues
Allysine residues are made by action of Lysyl oxidase acting on particular lysine and hydroxylysine residues
