DNA techniques

Question 1

narcolepsy

Answer 1

condition characterized by sudden and uncontrollable attacks of sleep

Question 2

restriction endonucleases

Answer 2

cuts the DNA of the phage into fragments

Question 3

protected by methylation

Answer 3

bacterial DNA

Question 4

isolated from ecoli

Answer 4

ECO R1

Question 5

Gel elctrophoresis run from one charge to the other

Answer 5

from negative to positive

Question 6

size of molecules on a gel electropherisis

Answer 6

from larger to smaller molecules

Question 7

SNoW DRoP

Answer 7

southern blot: DNA
Northern blot: RNA
Western blot: Proteins

Question 8

In a southern blot, duplex DNA needs to be turned into

Answer 8

single stranded DNA

Question 9

southern blot, hybrydization is succesful

Answer 9

when the DNA fragment that we want appears in the landmark according to its size

Question 10

Detection of a _____________ using Southern Blot

Answer 10

deletion

Question 11

Detecting ____________ _______ Via Southern Blot

Answer 11

trinucleotide expansion

Question 12

CGG repeat region

Answer 12

Fragile X

Question 13

Trinucleotide expansion has a greater molecular weight as severity increases

Answer 13

southern blot

Question 14

all individuals has the same mutation

Answer 14

sickle cell anemia

Question 15

detection of the _______ _______ associated with sickle cell anemia using ____________ ________

Answer 15

point mutation

southern blot

Question 16

single point mutation A–> T

Answer 16

sickle cell anemia

Question 17

molecular weight changes from 1.1 to 1.3 in a southern blot

Answer 17

detection of sickle cell anemia

Question 18

polymorphisms serve as

Answer 18

DNA fingerprinting

Question 19

differences in polymorphisms

Answer 19

restriction fragment length polymorhism

Question 20

altered RFLP

Answer 20

associated with possible mutation and diseases

Question 21

FISH

Answer 21

Fluorescence in situ hybridization

Question 22

to check for the presence, absence or copy member of the chromosome

Answer 22

FISH

Question 23

____ is separated according to size by electrophoresis through denaturing gel and transferred to a solid support

Answer 23

RNA

Question 24

SDS wash

Answer 24

Western blots–> protein neutralizing the charge

Question 25

antibodies are used for specificity

Answer 25

western blot

Question 26

DNA band shifts

Answer 26

to recognize DNA that are bound to protein

Question 27

used to establish where a given protein binds to DNA

Answer 27

DNA footprinting

Question 28

allows one to use the PCR technique to amplify RNA

Answer 28

RT-PCR

Question 29

Amplify DNA

Answer 29

PCR

Question 30

EcoRI makes sticky ends, foreign DNA inserted and then use DNA ligase to join the fragments

Answer 30

DNA cloning

Question 31

constructed directly from fragments of genomic DNA

Answer 31

genomic library

Question 32

derived from mRNA; dthe mRNA is converted to DNA by reverse transcriptase and then cloned

Answer 32

cDNA library

Question 33

single stranded DNA can potentially hybridize to any DNA molecule that contains complementary sequences

Answer 33

Heat denaturation

Question 34

After cooling, two different oligonucleotides (primers) are annealed to their complementary sequences sin the denatured DNA

Answer 34

Annealing

Question 35

annealed primers by a heat stable DNA polymerase (dNTPS are also required)

Answer 35

extension

Question 36

RT-PCR based test that predicts the likelihood of cancer recurrence

Answer 36

oncotype DX

Question 37

single strand confirmation polymorphism

Answer 37

confirmational difference between nucleotide sequences.

Question 38

involves sequencing of genome fragments that are on a chip allowing an entire genome to be sequenced at once

Answer 38

next generation sequencing

Question 39

chain termination DNA sequencing method uses _________ to chain terminate during DNA _____ and typically _____ dyes are used

Answer 39

uses dideoxynucleotide to chain terminate during replication using fluorescent dyes

Question 40

fragments of the genome

Answer 40

genomic library

Question 41

derived from mRNA being expressed in a given tissue

Answer 41

cDNA

Question 42

used when a mutation changes the folded structure of single stranded DNA as measured by gel-elec.

Answer 42

single strand confirmation