PRELIM 01 - Microscopy Flashcards
Equivalent of meter to inches
39.4 inches
The sizes of bacteria and protozoa are usually expressed in terms of __________
micrometers (µm)
A typical spherical bacterium (coccus) is approximately __________ in diameter
1 µm
A typical rod-shaped bacterium (bacillus) is approximately __________ wide × __________ long.
1 µm; 3 µm
Is an instrument used to see objects that are too small for the naked eye
Microscope
The science of investigating small objects using such an instrument
Microscopy
Means invisible to the eye unless aided by a microscope
Microscopic
Microscope that uses single lens
Simple light microscope
Microscope that uses set lenses or a lens system
Compound light microscope
Parts used for adjustments and support
Mechanical parts
Parts used to enlarge the specimen
Magnifying parts
Parts used to provide the light
Illuminating parts
Ability of the lens to distinguish two objects that are close together
Resolution
A function of objective lenses and its ability to gather light
Numerical aperture
2 components of numerical aperture
n, sinθ
Refractive index of the medium the lens is working in (Components of numerical aperture)
n
Measurement of the cone light that enters the objective (Components of numerical aperture)
sinθ
Resolving power - Compound microscope
200 nanometers
Resolving power - Scanning electron microscope
10 nanometers
Resolving power - Transmission electron microscope
0.2 nanometers
Degree of enlargement; no of times the length, breadth, or diameter of an object is multiplied
Magnification
Useful magnification
500x-1000x
Empty magnification
1400x and beyond
Occurs when the image continues to be enlarged, but no additional details are resolved
Empty magnification
Formula for magnification
Objective lens x Eyepiece lens