MIDTERM 01 - Culture Media Flashcards
Refers to food materials or substances required for growing microorganisms (In vivo)
Culture media/Growth media
Refers to the growth of microorganisms
Culture
Media where agar is the most commonly used solidifying agent (Media classifications based on physical state)
Solid media
Is a golden-yellow granular powder prepared from seaweeds that is not affected by the growth of bacteria
Agar
Media with a lower agar concentration; useful in the cultivation of microaerophilic bacteria (Media classifications based on physical state)
Semi-solid media
Media referred to as “broth”; bacteria grow uniformly producing general turbidity (Media classifications based on physical state)
Liquid media
Media that cultivates anaerobic bacteria at low oxygen, reducing oxidation-reduction potential (Media classifications based on the presence and absence of oxygen)
Anaerobic media
Media where it is easy to cultivate microbes on solid media and is used for keeping cultures in incubator (Media classifications based on the presence and absence of oxygen)
Aerobic media
Only aerobic growth; oxygen is required (Types of bacteria according to oxygen requirement)
Obligate aerobes
Both aerobic and anaerobic growth; has greater growth in the presence of oxygen (Types of bacteria according to oxygen requirement)
Facultative anaerobes
Only anaerobic growth; growth ceases in the presence of oxygen (Types of bacteria according to oxygen requirement)
Obligate anaerobes
Only anaerobic growth, but growth continues in the presence of oxygen (Types of bacteria according to oxygen requirement)
Aerotolerant anaerobes
Only aerobic growth; oxygen required in low concentrations (Types of bacteria according to oxygen requirement)
Microaerophiles
Growth occurs only where high concentrations of oxygen have diffused into the medium (Types of bacteria according to oxygen requirement)
Obligate aerobes
Growth is best where most oxygen is present, but occurs throughout the tube (Types of bacteria according to oxygen requirement)
Facultative anaerobes
Growth occurs only where there is no oxygen (Types of bacteria according to oxygen requirement)
Obligate anaerobes
Growth occurs evenly; oxygen has no effect (Types of bacteria according to oxygen requirement)
Aerotolerant anaerobes
Growth occurs only where a low concentration of oxygen has diffused into the medium (Types of bacteria according to oxygen requirement)
Microaerophiles
A container into which a lit candle is introduced before sealing the container’s airtight lid
Candle jar
General purpose media that supports growth of non-fastidious microbes; primarily used for the isolation of microorganisms (Media classifications based on nutritional factors)
Simple media (Basal media)