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[2ND YEAR] CCP 5.1 - Pharmaceutical Microbiology & Parasitology (Lab) > MIDTERM 01 - Culture Media > Flashcards

MIDTERM 01 - Culture Media Flashcards

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Biology 101 flashcards
1
Q

Refers to food materials or substances required for growing microorganisms in vitro (outside the body)

A

Culture media/Growth media

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2
Q

Refers to the growth of microorganisms

A

Culture

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3
Q

Media where agar is the most commonly used solidifying agent (Media classifications based on physical state)

A

Solid media

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4
Q

Is a golden-yellow granular powder prepared from seaweeds that is not affected by the growth of bacteria

A

Agar

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5
Q

Media with a lower agar concentration; useful in the cultivation of microaerophilic bacteria (Media classifications based on physical state)

A

Semi-solid media

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6
Q

Semi-solid media is useful in the cultivation of __________ bacteria

A

Microaerophilic

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7
Q

Media referred to as “broth”; bacteria grow uniformly producing general turbidity (Media classifications based on physical state)

A

Liquid media

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8
Q

2 examples of liquid media (NP)

A

Nutrient broth, Peptone solution

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9
Q

Only aerobic growth; oxygen is required (Types of bacteria according to oxygen requirement)

A

Obligate aerobes

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10
Q

Both aerobic and anaerobic growth; has greater growth in the presence of oxygen (Types of bacteria according to oxygen requirement)

A

Facultative anaerobes

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11
Q

Only anaerobic growth; growth ceases in the presence of oxygen (Types of bacteria according to oxygen requirement)

A

Obligate anaerobes

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12
Q

Only anaerobic growth, but growth continues in the presence of oxygen (Types of bacteria according to oxygen requirement)

A

Aerotolerant anaerobes

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13
Q

Only aerobic growth; oxygen required in low concentrations (Types of bacteria according to oxygen requirement)

A

Microaerophiles

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14
Q

Growth occurs only where high concentrations of oxygen have diffused into the medium (Types of bacteria according to oxygen requirement)

A

Obligate aerobes

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15
Q

Growth is best where most oxygen is present, but occurs throughout the tube (Types of bacteria according to oxygen requirement)

A

Facultative anaerobes

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16
Q

Growth occurs only where there is no oxygen (Types of bacteria according to oxygen requirement)

A

Obligate anaerobes

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17
Q

Growth occurs evenly; oxygen has no effect (Types of bacteria according to oxygen requirement)

A

Aerotolerant anaerobes

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18
Q

Growth occurs only where a low concentration of oxygen has diffused into the medium (Types of bacteria according to oxygen requirement)

A

Microaerophiles

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19
Q

A container into which a lit candle is introduced before sealing the container’s airtight lid

A

Candle jar

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20
Q

General purpose media that supports growth of non-fastidious microbes; primarily used for the isolation of microorganisms (Media classifications based on ingredients)

A

Simple media (Basal media)

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21
Q

2 examples of simple media/basal media (NN)

A

Nutrient broth, Nutrient agar

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22
Q

Media that has ingredients wherein the exact components are difficult to estimate (Media classifications based on ingredients)

A

Complex media

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23
Q

Specifically prepared media from pure chemical substances used for research purposes; the composition of every component is well known (Media classifications based on ingredients)

A

Synthetic/Defined media

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24
Q

Example of synthetic/defined media

A

Peptone water

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25
Q

Substances like blood, serum, and egg are added to the simple medium; used to grow bacteria that are exacting in their nutritional needs (Types of special media)

A

Enriched media

26
Q

Example of fastidious microorganism

A

Neisseria gonorrhoeae

27
Q

2 examples of enriched media (BC)

A

Blood agar, Chocolate agar

28
Q

Agar that contains mammalian blood used to isolate fastidious organisms and detect hemolysis (Examples of enriched media)

A

Blood agar

29
Q

Agar that contains red blood cells that have been lysed; used for growing fastidious bacteria (Examples of enriched media)

A

Chocolate agar

30
Q

Shows complete lysis of red blood cells resulting in complete clearing around the colonies (Types of Streptococci hemolysis)

A

Beta hemolysis

31
Q

Shows incomplete lysis of red blood cells, producing a greenish discoloration of the blood agar around the colonies (Types of Streptococci hemolysis)

A

Alpha hemolysis

32
Q

No hemolysis; results in no change in the medium (Types of Streptococci hemolysis)

A

Gamma hemolysis

33
Q

An inhibitory substance is added to a solid media to inhibit commensal or contaminating bacteria such as antibiotics, dyes, chemicals, and alteration of pH (Types of special media)

A

Selective media

34
Q

Media used selectively for Neisseria gonorrhoeae (Examples of selective media)

A

Thayer Martin media

35
Q

Antibiotic that is able to kill most gram positive organisms (Antibiotics found in Thayer Martin media)

A

Vancomycin

36
Q

Antibiotic that is added to kill most gram negative organisms except Neisseria (Antibiotics found in Thayer Martin media)

A

Colistin

37
Q

Can kill most fungi (Antibiotics found in Thayer Martin media)

A

Nystatin

38
Q

Inhibits gram negative organisms, especially swarming Proteus (Antibiotics found in Thayer Martin media)

A

Trimethoprim

39
Q

Media that is selective for gram negative bacteria; the dye methylene blue in the media inhibit the growth of gram positive bacteria (Examples of selective media)

A

Eosin methylene blue (EMB) media

40
Q

Media that is used for the isolation of Camphylobacter jejuni from fecal or rectal swabs; contains bacteriological charcoal, cefoperazone, and amphotericin B (Examples of selective media)

A

Camphylobacter agar

41
Q

3 components of Camphylobacter agar (BAC)

A

Bacteriological charcoal, Amphotericin B, Cefoperazone

42
Q

Is a solid media used for Mycobacterium tuberculosis; contains penicillin, nalidixic acid, and malachite green (Examples of selective media)

A

Lowenstein-Jensen media

43
Q

3 components of Lowenstein-Jensen media (PNM)

A

Penicillin, Nalidixic acid, Malachite green

44
Q

Media that are designed in such a way that different bacteria can be recognized on the basis of their colony color (Types of special media)

A

Differential media

45
Q

Media that distinguishes between lactose fermenters and non lactose fermenters (Types of differential media)

A

MacConkey media

46
Q

Lactose fermenters produce __________ colonies

A

Pink

47
Q

Non lactose fermenters produce __________ colonies

A

Colorless

48
Q

Media that distinguished between mannitol and non mannitol fermenters (Types of differential media)

A

Mannitol salt agar

49
Q

Mannitol fermenters include __________

A

Staphylococcus aureus

50
Q

Non-mannitol fermenters include __________

A

Staphylococcus epidermidis

51
Q

Positive growth but non-mannitol fermenters include __________

A

Micrococcus luteus

52
Q

Negative growth includes __________ and ___________ (EP)

A

Escherichia coli, Pseudomonas aeruginosa

53
Q

Media used for the isolation of Salmonella and Shigella spp. from clinical specimens and food samples (Types of differential media)

A

Xylose lysine deoxycholate (XLD) agar

54
Q

Media used for cultivation of pathogen from urine specimen and to inhibit swarming of Proteus sp. (Types of differential media)

A

Cysteine lactose electrolyte deficient (CLED) agar

55
Q

Media that is highly selective for the isolation of Vibrio cholerae and Vibrio parahaemolyticus (Types of differential media)

A

Thiosulfate-citrate-bile salts-sucrose (TCBS) agar

56
Q

Media used for transporting the samples (Types of special media)

A

Transport media

57
Q

2 examples of transport media (SB)

A

Stuart’s media, Buffered glycerol saline

58
Q

These media are used to grow anaerobic organisms (Types of special media)

A

Anaerobic media

59
Q

2 examples of anaerobic media (RT)

A

Robertson’s cooked meat media, Thioglycolate broth media

60
Q

Agar used for isolation and cultivation of fungi

A

Sabouraud dextrose agar (SDA)

61
Q

Agar used to promote growth and differentiation of plant cells

A

Marushige and Skoog medium (MSM)

62
Q

Agar used for plant cell suspension and callus cultures

A

Gamborg’s B5 medium

[2ND YEAR] CCP 5.1 - Pharmaceutical Microbiology & Parasitology (Lab) (8 decks)

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