prelab3quiz

Question 1

What type of protein is encoded by the gene for PTC perception?

contractile proteins

receptor protein

enzyme

cytosolic protein

a storage protein

Answer 1

receptor protein

Question 2

In this module, you will use RFLP ( Restriction Fragment Length Polymorphism) analysis to obtain your genotype for the PTC gene. What is the order of the four procedures required in this analysis?

DNA extraction - Restriction digest - Gel electrophoresis - PCR

PCR - Restriction digest - DNA extraction - Gel electrophoresis

PCR - Restriction digest - Gel electrophoresis - DNA extraction

DNA extraction - PCR - Restriction digest - Gel electrophoresis

Answer 2

DNA extraction - PCR - Restriction digest - Gel electrophoresis

Question 3

What type of SNP (substitution, insertion, deletion) is the most likely to be deleterious to protein function?

substitution

deletion

insertion

both deletion and insertion

both substitution and insertions

both substitutions and deletions

Answer 3

both deletion and insertion

Question 4

Where can you find DNA in a eukaryotic animal cell?

nucleus

chloroplast

mitochondria

ribosomes

both nucleus and mitochondria

both nucleus and chloroplasts

both chloroplasts and mitochondria

Answer 4

both nucleus and mitochondria

Question 5

Why is it so easy to get DNA from cheek cells?

because cheek cells do not divide

because these cells divide daily so old cells are always falling off

because they have larger cells

because their cells contain more DNA

Answer 5

because these cells divide daily so old cells are always falling off

Question 6

The _______solution is required to break open the cheek cells and the _________ solution is required to precipitate the DNA

proteinase K; alcohol

lysis solution; alcohol

saline; lysis

TE; lysis

Answer 6

lysis solution; alcohol

Question 7

If you wanted to isolate a copy of the gene that codes for a protein found in the liver you would need to isolate DNA from liver cells

True
False

Answer 7

False

Question 8

What is the purpose of the Proteinase K found in the lysis solution?

It allows the DNA molecules to clump together

It destroys all the proteins in the cell

It is responsible for degrading the histone proteins that wrap the DNA

Proteinase K is not found in lysis buffer

Answer 8

It is responsible for degrading the histone proteins that wrap the DNA

Question 9

Complete the animation on DNA extraction. In this protocol, the centrifuge is used twice. After the first centrifugation, the pellet contains the DNA and the supernatant contains the cell debris. After the second centrifugation, the pellet contains the cell debris and supernatant contains the DNA

True
False

Answer 9

false

Question 10

how should microfuge tubes be arranged for centrifugation?

Answer 10

they must be evenly balanced