prelab quiz 6 Flashcards
Which of the following procedures will allow us to confirm that we have successfully amplified the desired sequence of the COI gene?
DNA extraction
PCR purfication
Gel electrophoresis
Sanger Sequencing
Gel electrophoresis
The PCR purification step will cleanup the amplified PCR product using a reagent called ExoSAP-IT. How does this reagent clean the amplified PCR product? (choose all that apply)
It dephosphorylates unused nucleotides
It inactivates the polymerase enzyme
It hydrolyzes excess primers
It interferes with the DNA sequencing
It denatures the template DNA
It dephosphorylates unused nucleotides
It hydrolyzes excess primers
The ExoSAP-IT reaction occurs in two incubations that will be done using the thermocycler. Choose the two incubations that will be performed during the cleanup process
incubation at 80oC to inactivate the enzyme ExoI and SAP enzymes
incubation at 37oC to digest only the primers
incubation at 80oC to dephosphorylate the nucleotides
incubation at 37oC to inactivate the Exo enzyme
incubation at 37oC to digest the primers and nucleotides
incubation at 80oC to inactivate the enzyme ExoI and SAP enzymes
incubation at 37oC to digest the primers and nucleotides
Why must DNA amplified by PCR be cleaned up prior to sequencing?
To remove template DNA that can interfere with the sequencing reaction
to allow the Taq polymerase to bind to the template
To avoid contamination with the template
To remove excess primers and nucleotides that can interfere with sequencing
To remove excess primers and nucleotides that can interfere with sequencing
What is the expected size of the amplified sequence of the COI gene?
648 bp
800 bp
64 bp
it depends on the animal sample
648bp
If you do not see a band on the agarose gel, should you assume that you have no PCR product? Why?
Yes because if there was a PCR product, we would see the band
No because the agarose gel is not sensitive to low concentrations of DNA
Yes because if there is no visible band then there is no amplified DNA
No because the PCR product is always visible on an agarose gel
No because the agarose gel is not sensitive to low concentrations of DNA
Some seeds need light to germinate, such as celery, dill, lettuce, and petunias. Other seeds prefer only a shallow layer of soil, such as onions and pansies. Think of the experiment you will be performing in this lab. You will be testing the effect of light on seed germination. You have chosen two model organisms to test this effect.
The question asked here is how does light affect seed germination?
Based on the paragraph above, which is NOT a good hypothesis for the onion seeds (Allium cepa) and the lettuce seeds (Lactuca sativa)
Lettuce seeds prefer light environment for germination
Onion seeds will germinate better in high humidity
Lettuce seeds will have a higher germination rate when exposed to light
Light will have no effect on onion seed germination
Onion seeds will germinate better in the dark
Onion seeds will germinate better in high humidity
What are the two lights that are most important in regulating plant development
green
orange
blue
yellow
red
blue and red
In the experiment for lettuce seed germination, what is the independent variable
type of light
amount soaked filter papers
% seed germinated
number of seeds
type of seeds
type of light
In the two experiments you will be performing, what are the controlled variables? (Choose all that apply)
amount of water
number of seeds
type of seeds
seeds germinated
temperature
light
amount of water
number of seeds
type of seeds
