prelab quiz 5

Question 1

Which of the following is NOT a purpose of DNA barcoding?

detection of food fraud

identifying potential biological contraband

identifying new species

determination of the age of a new species

Answer 1

determination of the age of a new species

Question 2

Which is NOT a reason that makes the CO1 gene an ideal barcoding gene?

it displays low divergence between species and high level of divergence within species

it is a mitochondrial gene, so it has a large copies per cell so easily amplified

it is a short and highly variably region of the genome

It’s present in many living things

Answer 2

it displays low divergence between species and high level of divergence within species

Question 3

DNA barcoding uses universal barcodes that allow us to obtain the identity of many species

True
False

Answer 3

false (there is no universal barcode)

Question 4

The correct sequence, from the most to the least comprehensive, of the taxonomic levels listed here is __.

phylum, kingdom, order, class, species, family, and genus

phylum, family, class, order, kingdom, genus, and species

kingdom, phylum, order, class, family, genus, and species

kingdom, phylum, class, order, family, genus, and species

Answer 4

kingdom, phylum, class, order, family, genus, and species

Question 5

Phylogeny is only a hypothesis because we do not know the exact branching pattern of a phylogenetic tree because humans were not there to record what happened

True
False

Answer 5

true

Question 6

DNA barcoding relies on sequences that have retained enough sequence conservation through evolutionary history that it can be identified in many organisms, but that also has enough sequence diversity to differentiate organisms. What type of sequences do we use in barcoding experiments?

short sequences

highly variable sequences

sequences that display low divergence within species

all of the above

Answer 6

all of the above

Question 7

In this lab, what is the gene are we going to amplify?

Cytochrome c Oxidase subunit I

Coenzyme Q – cytochrome c reductase

Cytochrome b Oxygenase I

None of the above

Answer 7

Cytochrome c Oxidase subunit I

Question 8

A single mitochondrial gene A is more abundant that a single nuclear gene B because there are more mitochondria in the cell
True
False

Answer 8

true

Question 9

In this new module, you will use DNA barcoding to identify different unknown animal samples and determine phylogenetic relationship between these species. Order the steps required to obtain a phylogenetic tree using DNA barcoding

Analysis and sequence comparison
Purification of the amplicon
Collection of animal tissues
Sequencing of the barcode gene
Extraction of DNA from tissues
Amplification of the barcode gene

Answer 9

6
4
1
5
2
3

Question 10

During the DNA extraction from animal tissues, what is the function of the silica resin?

breaks up cell membranes and other materials

it binds to the nucleic acids

denatures enzymes that can degrade the purified DNA

removes contaminants from the sample

Answer 10

it binds to the nucleic acids