prelab 4 bio lab Flashcards
Which of the following statements about agarose gel electrophoresis is False?
Electrophoresis in agarose is a common way to separate DNA molecules according to size.
Ethidium bromide can be used to detect DNA.
Under the influence of the electrical field, positively charged DNA will migrate towards the negative charged end of the gel.
None of them
Under the influence of the electrical field, positively charged DNA will migrate towards the negative charged end of the gel.
During gel electrophoresis, large DNA fragments will ________ small DNA fragments.
migrate more rapidly than
separate into
migrate at the same speed as
cause degradation of
migrate more slowly than
migrate more slowly than
Suppose Jennifer has a PCR product that contains three DNA fragments of 300, 800, and 900 base pairs in length. She separates the three fragments using gel electrophoresis. Which fragment will be located close to the top of the gel?
the 300 bp fragment
the 800 bp fragment
the 900 bp fragment
All fragments will migrate the same distance
the 900 bp fragment
n gel electrophoresis, the Molecular Weight Marker or DNA ladder is useful:
For showing the size of the genomic DNA
For allowing you to estimate the size of any DNA band in other lanes on the same gel
For showing that DNA can stain orange with ethidium bromide
For telling you when to stop the electrophoresis experiment
For allowing you to estimate the size of any DNA band in other lanes on the same gel
When running an agarose gel, what is the purpose of the loading buffer (or loading dye)?
to bind to the DNA and make it fluoresces
to make the sample run faster
to visualize the DNA bands
to allow the sample to sink into the wells
to allow the sample to sink into the wells
These are steps taken in carrying out the PCR reaction:
- Attaching of primers by cooling
- Denaturation of strands
- DNA synthesis
- Heating
Which is the correct order? (from starting to ending of the reaction)
1, 2, 3, 4
2, 1, 3, 4
4, 3, 2, 1
4, 2, 1, 3
4, 2, 1, 3
Why is Taq polymerase used as the DNA polymerase in the polymerase chain reaction (PCR)?
it is able to copy a target DNA sequence without the use of DNA primers as a replication starting point
it can replicate much longer sequences of DNA than other types of DNA polymerases
it is less prone to making replication errors than DNA polymerases from other species
it is thermodynamically stable at the temperature used to separate the DNA strands
it is thermodynamically stable at the temperature used to separate the DNA strands
What is the size (in bp) of the region of the TAS2R38 gene we are amplifying in this lab
239 bp
64 bp
303 bp
1,143 bp
303 bp
Look at the sequence of TAS2R38. The numbers at the beginning of each row indicate the nucleotide position. After 10 nucleotides, there is a space. How many Fnu4H1 sites do you find in this sequence? (in other words, how many times will this enzyme cut this sequence?)
1 atgttgactc taactcgcat ccgcactgtg tcctatgaag tcaggagtac atttctgttc
61 atttcagtcc tggagtttgc agtggggttt ctgaccaatg ccttcgtttt cttggtgaat
121 ttttgggatg tagtgaagag gcaggcactg agcaacagtg attgtgtgct gctgtgtctc
181 agcatcagcc ggcttttcct gcatggactg ctgttcctga gtgctatcca gcttacccac
241 ttccagaagt tgagtgaacc actgaaccac agctaccaag ccatcatcat gctatggatg
301 attgcaaacc aagccaacct ctggcttgct gcctgcctca gcctgcttta ctgctccaag
361 ctcatccgtt tctctcacac cttcctgatc tgcttggcaa gctgggtctc caggaagatc
421 tcccagatgc tcctgggtat tattctttgc tcctgcatct gcactgtcct ctgtgtttgg
481 tgctttttta gcagacctca cttcacagtc acaactgtgc tattcatgaa taacaataca
541 aggctcaact ggcagaataa agatctcaat ttattttatt cctttctctt ctgctatctg
601 tggtctgtgc ctcctttcct attgtttctg gtttcttctg ggatgctgac tgtctccctg
661 ggaaggcaca tgaggacaat gaaggtctat accagaaact ctcgtgaccc cagcctggag
721 gcccacatta aagccctcaa gtctcttgtc tcctttttct gcttctttgt gatatcatcc
781 tgtgttgcct tcatctctgt gcccctactg attctgtggc gcgacaaaat aggggtgatg
841 gtttgtgttg ggataatggc agcttgtccc tctgggcatg cagccatcct gatctcaggc
901 aatgccaagt tgaggagagc tgtgatgacc attctgctct gggctcagag cagcctgaag
961 gtaagagccg accacaaggc agattcacac tgtgctga
Reminder Fnu4H1 recognition site is:
5’-GCNGC-3’
3’-CGNCG-5’
The * indicates the cut site and N indicates any nucleotide
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