Prelab 1 Quiz Flashcards
To read the absorbance of a sample, what button must you press?
nm
Incorrect Response
A/T/C
0 ABS 100%T.
None, the reading is automatic
None, the reading is automatic
When using frosted or ridged cuvettes, how should you handle the sample cuvettes (which side should you touch with your fingers, and which should you avoid)?
touch the clear side
avoid the ridge side
touch both sides
avoid the clear side
avoid the clear side
Which button on the machine is used to set the blank?
A/T/C
0 ABS 100%T
nm
utility
0 ABS 100%T
What dye is used to measure the concentration of proteins in the Bradford Assay we will perform?
BPB (Bromophenol blue)
BSA (Bovine Serum Albumin)
Methylene Blue
Coomassie blue
Coomassie blue
When loading the cuvette in the chamber of the spectrophotometer, the arrow on the cuvette should face the direction of the light
True False
true
When measuring absorbance of a sample, what does the blank contain?
the solute
any other liquid
the solvent
the sample tested
the solvent
You have a stock solution of protein that you would like to dilute. How much stock protein do you need to add to 800 μL of buffer to make a 1:5 dilution?
200ul
500ul
100ml
100ul
200ul
You have been given a stock solution of dye that has a 10X concentration. You wish to make 1000ul of a 1X dilution of this dye. Give the volume of water that you will use (in ul)
900ul use cc1v1=c2v2
You have been given a stock solution of dye that has a 10X concentration. You wish to make 1000ul of a 1X dilution of this dye. Give the volume of 10X dye that you will use (in ul)
100ul
C1V1 = C2V2
V1 = C2V2/C1 = (1X x 1000ul)/10X = 100ul
The rest will be water (900ul)
In a Bradford Assay, when absorbance increases, the protein concentration decreases
True False
false
