Pre-lab wk1

Question 1

Which enzyme is used to convert RNA to DNA?

Answer 1

Reverse transcriptase

Question 2

What are the steps of the PCR cycle and the temperature?

Answer 2

1. Denaturation (95 degrees)
   2.Annealing (55 degrees)
2. Extension (72 degrees)

Question 3

What is the size of PCR products A and B?

Answer 3

200 bp and 900 bp respectively for the example pic

(But for the MCQ count the line that levels up with A and the line that levels up with B starting from below)

Question 4

What is PCR

Answer 4

PCR is a process for the amplification of specific fragments of DNA.

Question 5

What is Real time PCR

Answer 5

Real-Time PCR is a specialized technique that allows a PCR reaction to be visualized “in real time” as the reaction progresses

Question 6

What is Real-Time PCR used for?

Answer 6

Gene expression analysis
-Medical research & Drug research

Disease diagnosis
- Viral quantification (HIV)

Food testing
-GMO food

Transgenic research
- Gene copy number

Question 7

How do We Measure DNA in a PCR Reaction?

Answer 7

Use reagents such as SYBR green I dye that “fluoresce” in the presence of amplified DNA

They bind to double stranded DNA and emit light when illuminated with specific wavelength

Question 8

Which of the following pathogens can be detected and identified using qPCR?

Answer 8

Any pathogen type

Question 9

What Type of Instruments are used withReal-Time PCR?

Answer 9

1.Thermal cycler (PCR machine)

2.Optical Module ( to detect florescence in the tubes during run)

3.Computer (to translate the fluorescence data into meaningful results)