PCR MCQ

Question 1

which of the following is basic requirement of PCR reaction?

(a) Two oligonucleotide primers

(b) DNA segment to be amplified

(c) A heat-stable DNA polymerase

(d) All of the above

Answer 1

D) all of the above

Question 2

Primers used for the process of PCR are

(a) Single-stranded RNA oligonucleotide

(b) Single-stranded DNA oligonucleotide

(c) Double-stranded RNA oligonucleotide

(d) Double stranded DNA oligonucleotide

Answer 2

(b) Single-stranded DNA oligonucleotide

Question 3

At what temperature does annealing of DNA and primer take place?

(a) 54°C
(b) 96°C
(c) 42°C
(d) 74°C

Answer 3

(a) 54°C

Question 4

Polymerase used for PCR is extracted from

(a) Homo sapiens

(b) Thermus aquaticus

(c) Escherichia coli

(d) Saccharomyces cerevisiae

Answer 4

(b) Thermus aquaticus

Question 5

What is the process of binding of primer to the denatured strand called?

(a) Annealing
(b) Renaturation
(c) Denaturation
(d) None of the above

Answer 5

(a) Annealing

Question 6

Denaturation is the process of?

(a) Heating between 72°C

(b) Heating between 40 to 60°C

(c) Heating between 90 to 98°C

(d) None of the above

Answer 6

(c) Heating between 90 to 98°C

Question 7

At what temperature does denaturation of DNA double helix takes place?

(a) 54°C
(b) 74°C
(c) 94°C
(d) 60°C

Answer 7

(c) 94°C

Question 8

How many DNA duplexes are obtained from one DNA duplex after 4 cycles of PCR?

(a) 8
(b) 4
(c) 32
(d) 16

Answer 8

(d) 16- The number of double stranded DNA pieces is doubled in each cycle, so that after n cycles you have 2^n (2 to the n:th power) copies of DNA. For example, after 4 cycles you have 16 copies

Question 9

Which of the following statements are true regarding PCR?

(a) Primer extension occurs at 72°C
(b) Denaturation involves heating at 90 to 98°C
(c) Annealing involves the binding of primer between 40 to 60C°C
(d) All of the above

Answer 9

(d) All of the above

Question 10

The polymerase chain reaction is…?

(a) It is a DNA sequencing technique.
(b) It is a DNA degradation technique
(c) It is a DNA amplification technique
(d) All of the above

Answer 10

(c) It is a DNA amplification technique

Question 11

Which of the following statements is accurate for the PCR - polymerase chain reaction?

(a) Automated PCR machines are called thermal cyclers

(b) A thermostable DNA polymerase is required

(c) Millions to billions of desired DNA copies can be produced from microgram quantities of DNA

(d) All of the above

Answer 11

(d) All of the above

Question 12

Thermus aquatics is the source of…?

(a) Vent polymerase
(b) Primase enzyme
(c) Tag polymerase
(d) Both a and c

Answer 12

(c) Tag polymerase

Question 13

Why are vent polymerase and Pfu more efficient than the Taq polymerase?

(a) Because of proofreading activity
(b) Because of more efficient polymerase activity
(c) Both a and b
(d) None of the above

Answer 13

(b) Because of more efficient polymerase activity

Question 14

Which of the following is the first and the most important step in the polymerase chain reaction?

(a) Annealing
(b) Primer extension
(c) Denaturation
(d) None of the above

Answer 14

(c) Denaturation

Question 15

In a PCR cycle, (1), (2) and (3) refer to:

(a) Elongation, denaturation and annealing respectively

(b) Denaturation, elongation and annealing respectively

(c) Elongation, annealing and denaturation respectively

(d) Denaturation, annealing and elongation respectively

Answer 15

(d) Denaturation, annealing and elongation respectively

Question 16

What is PCR?

Answer 16

Amplification of specific DNA fragment from complex pool of DNA

Question 17

What does the PCR help do?

Answer 17

Diagnoses disease, clone, sequences of genes and carry out studies

Question 18

What are PCR principles?

Answer 18

Ability of DNA polymerase to synthesise new strand of DNA complementary

Question 19

What are ingredients are needed for PCR?

Answer 19

Tax polymerase
Primers
Template DNA
Nucleotides

Question 20

What equipment is needed for PCR

Answer 20

Thermal cycler
Electrophoresis system

Question 21

What does PCR stand for ?

Answer 21

Polymerase Chain Reaction

Question 22

What does qPCR stand for?

Answer 22

Quantitative Polymerase Chain reaction

Question 23

what is qPCR?

Answer 23

determines the amount of PCR product present at a given cycle (Measuring DNA using PCR)

Question 24

What is qPCR principle?

Answer 24

the amount of amplification product is measured in each PCR cycle using fluorescence

Question 25

What equipment are needed for qPCR?

Answer 25

-Thermal cycler
-Light source
-Detector
- Tax polymerase
-dNTPs
-Primers
-Reverse transcriptase

Question 26

What are the methods of PCR?

Answer 26

(1) denaturation of the template into single strands; 92-98
(2) annealing of primers to each original strand for new strand synthesis; and 52-65
(3) extension of the new DNA strands from the primers. 72

Question 27

what happens in the annealing step of qPCR?

Answer 27

During the annealing step of a qPCR assay, the probe and PCR primers attach to their complementary sequences in the target region. Then, during primer extension or elongation, the exonuclease activity of DNA polymerase degrades the probe, releasing the 5’ fluorophore from the quencher.