PCR MCQ Flashcards
which of the following is basic requirement of PCR reaction?
(a) Two oligonucleotide primers
(b) DNA segment to be amplified
(c) A heat-stable DNA polymerase
(d) All of the above
D) all of the above
Primers used for the process of PCR are
(a) Single-stranded RNA oligonucleotide
(b) Single-stranded DNA oligonucleotide
(c) Double-stranded RNA oligonucleotide
(d) Double stranded DNA oligonucleotide
(b) Single-stranded DNA oligonucleotide
At what temperature does annealing of DNA and primer take place?
(a) 54°C
(b) 96°C
(c) 42°C
(d) 74°C
(a) 54°C
Polymerase used for PCR is extracted from
(a) Homo sapiens
(b) Thermus aquaticus
(c) Escherichia coli
(d) Saccharomyces cerevisiae
(b) Thermus aquaticus
What is the process of binding of primer to the denatured strand called?
(a) Annealing
(b) Renaturation
(c) Denaturation
(d) None of the above
(a) Annealing
Denaturation is the process of?
(a) Heating between 72°C
(b) Heating between 40 to 60°C
(c) Heating between 90 to 98°C
(d) None of the above
(c) Heating between 90 to 98°C
At what temperature does denaturation of DNA double helix takes place?
(a) 54°C
(b) 74°C
(c) 94°C
(d) 60°C
(c) 94°C
How many DNA duplexes are obtained from one DNA duplex after 4 cycles of PCR?
(a) 8
(b) 4
(c) 32
(d) 16
(d) 16- The number of double stranded DNA pieces is doubled in each cycle, so that after n cycles you have 2^n (2 to the n:th power) copies of DNA. For example, after 4 cycles you have 16 copies
Which of the following statements are true regarding PCR?
(a) Primer extension occurs at 72°C
(b) Denaturation involves heating at 90 to 98°C
(c) Annealing involves the binding of primer between 40 to 60C°C
(d) All of the above
(d) All of the above
The polymerase chain reaction is…?
(a) It is a DNA sequencing technique.
(b) It is a DNA degradation technique
(c) It is a DNA amplification technique
(d) All of the above
(c) It is a DNA amplification technique
Which of the following statements is accurate for the PCR - polymerase chain reaction?
(a) Automated PCR machines are called thermal cyclers
(b) A thermostable DNA polymerase is required
(c) Millions to billions of desired DNA copies can be produced from microgram quantities of DNA
(d) All of the above
(d) All of the above
Thermus aquatics is the source of…?
(a) Vent polymerase
(b) Primase enzyme
(c) Tag polymerase
(d) Both a and c
(c) Tag polymerase
Why are vent polymerase and Pfu more efficient than the Taq polymerase?
(a) Because of proofreading activity
(b) Because of more efficient polymerase activity
(c) Both a and b
(d) None of the above
(b) Because of more efficient polymerase activity
Which of the following is the first and the most important step in the polymerase chain reaction?
(a) Annealing
(b) Primer extension
(c) Denaturation
(d) None of the above
(c) Denaturation
In a PCR cycle, (1), (2) and (3) refer to:
(a) Elongation, denaturation and annealing respectively
(b) Denaturation, elongation and annealing respectively
(c) Elongation, annealing and denaturation respectively
(d) Denaturation, annealing and elongation respectively
(d) Denaturation, annealing and elongation respectively
What is PCR?
Amplification of specific DNA fragment from complex pool of DNA
What does the PCR help do?
Diagnoses disease, clone, sequences of genes and carry out studies
What are PCR principles?
Ability of DNA polymerase to synthesise new strand of DNA complementary
What are ingredients are needed for PCR?
Tax polymerase
Primers
Template DNA
Nucleotides
What equipment is needed for PCR
Thermal cycler
Electrophoresis system
What does PCR stand for ?
Polymerase Chain Reaction
What does qPCR stand for?
Quantitative Polymerase Chain reaction
what is qPCR?
determines the amount of PCR product present at a given cycle (Measuring DNA using PCR)
What is qPCR principle?
the amount of amplification product is measured in each PCR cycle using fluorescence
What equipment are needed for qPCR?
-Thermal cycler
-Light source
-Detector
- Tax polymerase
-dNTPs
-Primers
-Reverse transcriptase
What are the methods of PCR?
(1) denaturation of the template into single strands; 92-98
(2) annealing of primers to each original strand for new strand synthesis; and 52-65
(3) extension of the new DNA strands from the primers. 72
what happens in the annealing step of qPCR?
During the annealing step of a qPCR assay, the probe and PCR primers attach to their complementary sequences in the target region. Then, during primer extension or elongation, the exonuclease activity of DNA polymerase degrades the probe, releasing the 5’ fluorophore from the quencher.
