Practicals Flashcards
For the epigenetics prac, if the undigested control had two bands and the sample had one, what would we say?
Sample is female.
For the epigenetics prac, if the undigested control had no bands and the sample had no bands, what would we interpret?
PCR failed.
For the epigenetics prac, if the undigested control and the sample both had two bands, what would we interpret?
Digest failed.
X chromosome inactivation is an example of what system?
Dosage compensation system.
What chromosome is Hprt on?
The X chromosome.
Would Hprt’s promoter be methylated on the active or inactive X chromosome?
Inactive.
What gene served as a control in the epigenetics prac?
Gapdh.
Would promoter methylation be the same or different across the sexes for Gapdh?
The same (unmethylated).
Would the Hprt gene promoter be methylated if it is on the male X-chromosome?
No.
Will HpaII cut methylated or unmethylated CCGG?
Unmethylated.
The Hprt gene from the active X chromosome is not cut by HpaII and is thus amplified. True or false?
False.
If the HpaII digest failed, there would be no amplification of the Gapdh locus in both sexes. True or false?
False.
How many bands are expected in females if the HpaII digest is successful?
1.
What is the central difference between the three fingerprint types?
Number of triradii.
rbcL is the large subunit of which important protein?
RuBisCO
