Practical lab classes-blood

Question 1

What are the main sources of blood glucose?

Answer 1

Diet, glycogenolysis, and gluconeogenesis

Question 2

What is haematocrit a measure of?

Answer 2

% of erythrocytes in your blood/ a sample

Question 3

What can a HemoCue measure?

Answer 3

The g of haemoglobin per 100mL of blood

Question 4

What can be measured from a blood sample?

Answer 4

Blood glucose, blood lactate, blood haemoglobin and hematocrit

Question 5

What does OGTT stand for?

Answer 5

Oral glucose tolerance test

Question 6

What does an OGTT measure?

Answer 6

How well the body can process large amounts of sugar

Question 7

How is an OGTT done?

Answer 7

Fast for 8 hrs
Drink a drink containing 75g glucose (maltodextrin)
BGC measured every x time (e.g. 30 mins) after

Question 8

What is FPG?

Answer 8

Fasting plasma glucose

Question 9

What does FPG measure?

Answer 9

Blood glucose levels at least 8 hrs after fasting

Question 10

What does HBA1C stand for?

Answer 10

Haemoglobin A1C

Question 11

What does a HBA1C test measure?

Answer 11

Total amount of glucose attached to haemoglobin

Question 12

What is the healthy range of blood sugar?

Answer 12

4-7mmol/l

Question 13

What kind of blood sampling was done in the lab?

Answer 13

Finger-tip

Question 14

How can the hematocrit be measured?

Answer 14

Prick your finger with a lancet, collect blood using hematocrit tube (red end).
Non-red end is pressed into plasticine

Question 15

Equipment for measuring hematocrit?

Answer 15

Gloves, sharps and soft bin, tissue, alcohol swab, lancet and hematocrit tube.

Question 16

What is done to the finger before the lancet is applied?

Answer 16

Cleaned with an alcohol swab

Question 17

Where on the finger is the lancet applied?

Answer 17

Opposite to the nail bed

Question 18

What is done with the first drop of blood that is visible?

Answer 18

It is wiped away

Question 19

How should the participants hand be held?

Answer 19

Finger pointing down

Question 20

What end is the blood collected into?

Answer 20

Red end

Question 21

How full should the tube be?

Answer 21

2/3-3/4

Question 22

Which end of the tube is pressed into the plasticine?

Answer 22

Non-red end

Question 23

What equipment is required to measure haemoglobin

Answer 23

Gloves, tissue paper, alcohol swab, lancet, HemoCue microcuvette, HemoCue analyser, and a sharps and softs bin

Question 24

What temp should the subjects hand be when taking a fingertip sample?

Answer 24

Warm

Question 25

How is the blood collected into the microcuvette?

Answer 25

Present the opening of the microcuvette onto the dollop of blood–> the plate will fill

Question 26

What position should the subjects hand be in when filling the microcuvette?

Answer 26

Finger pointing downwards

Question 27

What is done with the microcuvette after the blood has been collected?

Answer 27

It is placed into the HemoCue analyser

Question 28

How does the HemoCue analyser work?

Answer 28

Place microcuvette in the machine, wait a bit and it will give you the haemoglobin conc

Question 29

What units does a HemoCue analyser use?

Answer 29

Grams/litre

Question 30

What is done with the hematocrit tube after the sample has been taken?

Answer 30

It is centrifuged for 10 minutes

Question 31

How is the haematocrit read once the haematocrit tube has been centrifuged?

Answer 31

It is placed in a micro-haematocrit reader

Question 32

How is a micro-haematocrit reader used once the tube is in?

Answer 32

Bottom of erythrocytes is aligned with 0 on the scale, reader plate is moved to plasma is aligned with top line and slider is moved to white line is aligned with top of erythrocytes

Question 33

Hematocrit of men?

Answer 33

40.7%-50.3%

Question 34

Hematocrit of women?

Answer 34

36.1% to 44.3%