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ppt Flashcards

1
Q

these are simple tests that can identify the bacterium up to a generic level

A

gram reaction

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2
Q

widely used for the routine staining of bacteria in smears

A

gram stain

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3
Q

useful for demonstrating dermatophilus congolensis, rickettsiae and borrelia species which stain blue

A

giemsa stain

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4
Q

especially useful for recognizing campylobacter species, brachyspira species and fusobacterium species which stain red

A

dilute carbol fuchsin

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5
Q

used for the identification of bacillus anthraic in blood smears

A

polychrome methylene blue

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6
Q

hot concentrated carbol fuchsin which penetrates mycobacterial cell walls is retained after acid alcohol decoloriation

A

ziehl-neelsen stain

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7
Q

unlike ziehl neelsen stain, this metjod employs dilute carbol fuchsin with decolorization by acetic acid

A

modified zhiel neelsen

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8
Q

macConkey may differ in the composition of ___

A

bile salts or presence of crystal violetu

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9
Q

supports the growth of all members of the eneterobacteriaceae but selectively inhibitory to tother gram negative bacteria

A

macconkey

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10
Q

allows differentiation of lactose fermenters from non-lactose fermenters

A

macconkey

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11
Q

this test detects enzyme catalase that converts hydrogen peroxide to water and gaseous oxygen

A

catalase test

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12
Q

the positive reaction of this test is the effervescence of oxygen gas within few seconds

A

catalase test

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13
Q

Routinely used as an aid in distinguishing between
staphylococcus from streptococcus

A

catalase test

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14
Q

in catalase test, which is positive. staphy or strepto

A

staphy

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15
Q

2 methods of catalase test

A
  1. Loopful of bacteria on clean
    microscope slide + a drop of
    3% hydrogen peroxide
  2. A drop of 3% hydrogen peroxide is added to a colony on the plate and another drop on the area of the agar plate without bacterial growth
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16
Q

this test depends on the presence of cytochrome c oxidase in a
bacterial cell

A

oxidase test

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17
Q

these are oxidase negative

A

anaerobes

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18
Q

these bacterai can be used as a positive control organism

A

pseudomonas aurUGINOSA

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19
Q

the majority of bacteria are motile by means of

A

flagella

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20
Q

Motility can be temperature –dependent and some bacteria tend to be motile at

A

ambient temp but not at 37 degree celsius

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21
Q

these can be added in the media to aid the detection of motility

A

tetrazolium salt

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22
Q

a composite medium for the determination of hydrogen sulphide and indole production and motility

A

SIM medium

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23
Q

medium used mainly for enterobacteriaceae

A

SIM medium

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24
Q

this test is used to determine the oxidative or fermentative metabolism of a carbohydrate by
the bacterium

A

oxidation-fermentation test

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25
Q

The medium is semi- solid and
usually contains glucose and bromothyl blue as
pH indicat

A

o-f test

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26
Q
  • Bacteria that requires atmospheric oxygen for
    growth and metabolism are
A

oxidative

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27
Q

Bacteria that can metabolize glucose under
aerobic or anaerobic conditions are

A

facultative anaerobes

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28
Q

facultative anaerobes in o-f test is

A

fermentative

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29
Q

o-f test results

A

if unreactive, both opena nd sealed tube is green (bordetella)
oxidation- open tube is yellow, sealed tube is green (pseudomonas)
in fermentation, open and sealed tube is both yellow(aeromonas)

30
Q

in lana test, a swab is impregnated with ____

A

l-alanine-4-nitroanilide

31
Q

what is the reslt if the swab in lana test turned yellow

A

the bacterium is gram negative

32
Q

medium to indicate hemolysis

A

blood agar

33
Q

what does it mean if a viscous gel is forming in a KOH test

A

it is a gram negative bacteria

34
Q

the majority of a gram positive bacteria is susceptible to

A

vancomycin. gram negative are resistant

35
Q

what are the primary biochemical tests for the identification of bacteria

A

gram staining
growth or absence of growth on MacConkey
presence or absence of hemolysis on blood agar
catalase test
oxidase test
motility test
SIM medium
KOH test
O-F test
LANA test
susceptibiluty to vancomycin

36
Q

enzymatic attack on sugar with
acid and gas production (Durham tubes

A

carbohydrate fermentation test

37
Q

tested for the ability of the microorganism to use citrate as sole carbon source

A

citrate utilization test

38
Q

chiefly used in the differentiation
of Gram-positive rod

A

citrate utilization test

39
Q

what bacteria can utilize citrate for growth

A

aerogenes group

40
Q

lacks transport system that
would permit the citrate to enter the cell to be use

A

e coli

41
Q

ability of an organism to liquefy
or hydrolyze gelatin

A

gelatin liquefaction

42
Q
A
43
Q

what does gelatin liqeufaction indicates

A

gelatin loses its gelling properties
and remains a liquid even at low
temperature

44
Q
  • a i d s i n t h e i d e n tifi c a ti o n o f
    o r g a n i s m s w i t h i n t h e
    Enterobacteriaceae
A

decarboxylase test

45
Q

The specific decarboxylase enzyme produced by an organism results in the breakdown of the amino acid concerned and the fermentation of the corresponding amino acid

A

ornithine-putrescine
lysine-cadaverine
arginine-agmatine
glutamic acid-aminobutyric acid

46
Q

tryptophan split to indole

A

indole test

47
Q

together with VP test, this is useful in
differentiating between coli-aerogenes
group of enteric bacter

A

methyl red test

48
Q

-it is a confirmatory test for the
butylene glycol type of fermentation of
bacteria

A

voges-prokauer test

49
Q

it detects the presence of acetoin or acetylmethylcarbinol, which is an intermediate in the production of butylene glycol

A

voges-prokauer test

50
Q

discuss the nitrate reduction test

A

nitrate-nitrite-nitrogen gas

51
Q

-test for the production of the
enzyme urease by bacteri

A

urease test

52
Q

-used for the identification of
Proteus organism as well as the
differentiation of other
microorganisms

A

urease test

53
Q

Urease breakdown urea to form

A

ammonia nad co2

54
Q

-fermentation of sugars, hydrogen sulfide production, gas formation (hydrogen and carbon dioxide)

A

triple sugar iron

55
Q

-useful for presumptive identification of
Salmonella

A

tsi

56
Q

in tsi, utilization of the ferrous substrate is indicated by

A

production of black color

57
Q

the black color in tsi is

A

hydrogen sulfide

58
Q

in tsi, if the medium becomes yellow, it means

A

acid production

59
Q

alkaline color of the medium in tsi is due to

A

peptone degradation

60
Q

in tsi, gas formation will show

A

cracks, bubbles, and indentation of the medium

61
Q

a basic medium where non fastidious bacteria grows

A

nutrient agar

62
Q

it is suitable for demonstrating colonial morphology and pigment production. also used for viable counting methods

A

nutrient agar

63
Q

an enriched medium which supports the growth of most pathogenic bacteria and is used for their primary isolation

A

blood agar

64
Q

allows the recognition of haemolysin production

A

blood agar

65
Q

allows differentiation of lactose fermenters and non lactose fermenters

A

macconkey agar

66
Q

selective enrichment media used for the isolation of salmonellae from samples containing other gram negative enteric organism

A

selenite broth, rappaport-vassiliadis broth

67
Q

a blood agar based selective medium used for the inocculation and recognition of streptococci

A

edwards medium

68
Q

heat treated blood agar which supplies special growth requirements for the isolation of heamophilus species and for the culture of taylorella equigenitalis

A

chocolate agar

69
Q

used for the identifiction of enterobacteriadceae

A

IMViC testh

70
Q

what is imvic test

A

indole
methylene red
voges-prokauer
citrate utilization

bacteriology (8 decks)

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