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bacteriology > gram pos part 2 > Flashcards

gram pos part 2 Flashcards

1
Q

nocardia asteroides in dogs disease

A

canine nocardiosis

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2
Q

nocardia asteroides in cattle

A

chronic mastitis and abortion

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3
Q

what causes bovine farcy in cattle

A

nocardia farcinica

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4
Q

what is the cahracteristic of nocardia on sabouraud dextrose agar

A

colonies are orange coloured and wrinkled

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5
Q

virulence factor of nocardi

A

mycolic acid, nocardial lipids and superoxide dismutase

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6
Q

pathogenesis of nocardia

A

transmitted through inhalation, ingestion, and skin wounds. infection begins as a nodule or pustule that ruptures and there will be suppuration with subsequent induration

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7
Q

it has the ability to survive within phagocytic vacuoles by preventing phagolysosome formation

A

nocardia

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8
Q

treatment of nocardia

A

surgical debridement and drainage of lesions
antimicrobials like trimethoprim

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9
Q

trimethoprim antimicrobials

A

sulfamethoxazole, sulfanamide, erythromycin

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10
Q

the only species in the genus nocardia, and maintain itself in small foci of infection on a carrier animal or material within scab particles in dust

A

dermatophilus congolensis

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11
Q

transmission of d. congolensis

A

direct contact, splashing effect of heavy rains, insect activity

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12
Q

virulence factor of d. congolensis

A

keratinolytic activity

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13
Q

an activity that resists phagocytosis by growing into aggregates or filaments sufficiently enough to discourage ingestion

A

keratinolytic

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14
Q

pathogenicity of d. congolensis

A

causes skin infection

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15
Q

disease caused by d. conglensis

A

strawberry footrot, lumpy wool or rainscald streptothricosis, dermatophilosis and grease heal

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16
Q

specimen of d. congolensis

A
  • a tuft of hair is plucked from the lesion with scab material adhering to it
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17
Q

what stain is used in d. congolensis

A

gram or giemsa

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18
Q

prevention of d. congolensis

A

grazing areas are cleared of throny scrub and tick infestation should be reduced by dipping to acaricides

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19
Q

treatment of d. congo

A

long acting oxytetracyclines, penicilllin, streptomyces

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20
Q

opportunistic pathogen of young foals with 2 morph. forms

A

rhodococcus equi

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21
Q

has mucoid salmon pink colonies

A

rhod

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22
Q

can survive and replicate intracellularly, mostly within mononuclear phagocytes

A

rhod

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23
Q

for persistent infection in severely immunodefficient mice

A

VApA

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23
Q

virulence factor of rhod

A

interference with endosomal maturation following phagocytosis and suppressing the acidification.
capsule
prod. of phospholipase c and cholesterol oxidase

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24
Q

Pathogenesi of Suppurative bronchopneumonia in foal

A

airosolized virulent R .equi inhaled through dust→ reached the lungs and alveoli→ survive and replicate inside
alveolar macrophages→ necrosis of macrophage→ pyogranulomatous lesions in the lungs

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25
Q

When cough up and swallowed in large numbers, the bacteria enter M cells of the intestine and cause pyogranulomas in
GALT and intestinal lymph nodes

A

rhod equi enteritis

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26
Q

specimen in rhod

A

tracheal wash or aspirates for culture

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27
Q

colonial morph of rhod

A

colonies are small, smooth,
shiny, and non- hemolytic after 24h of incubation,

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28
Q

colonial morph of rhod as it ages

A

larger, mucoid, salmon pink in color

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29
Q

in camp, it shows shovel effect against S. aureus; enhancement of staphylococcal beta
hemolysin

A

rhod

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30
Q

control and prevention of rhod

A

vaccination of vapA
removal of feces
disinfect the soil

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31
Q

catalase positive, oxidase negative, faculattive anaerobes

A

coryne and listeria

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32
Q

vir factor of corynebacterium pseudotuberculosis

A

attributed to the hemolytic toxin which has phospholipase activity to the cell
wall lipids

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33
Q

e pyogenic bacteria causing a variety of suppurative conditions

A

corynebacteria

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34
Q

transmission of c. pseudotuberculosis

A

contamination of shearing, docking wounds and through arthropods bites or contaminated dips

35
Q

characterized by the lamellar (“onion-ring”) appearance
containing yellow- green viscous pus with a toothpaste-like consistency

A

lymph node in corynebacteru=ium

36
Q

specimens of corynebacterium

A

pus or exudates, midstream urine

37
Q

(Chinese letter appearance

A

coryne

38
Q

isolation in coryne

A

sheep or ox blood agar

39
Q

colonial morph of c. bovis

A

small white dry non hemo

40
Q

colonial morph of c. kutscheri

A

small, whitish colonies that bear a resemblance to those of C.pseudotuberculosis. Occasional strains are
haemolytic

41
Q

colonial morph of c. pseudotuber

A

small, white, dry colonies surrounded by a narrow zone of haemolysis after 48-72h of incubation

42
Q

disease caused by c. bovis

A

subclinical mastitis in teat cistern

43
Q

c renale and pilosum in cattle disease

A

cystitis, pyelonerphritis

44
Q

treatment and control of coryne

A

since antibiotic is ineffective, culling or segregation and bacterin toxoid is reco

45
Q

small gram pos rods found in herbage and fresh water which may be present in feces and sewage effluent

A

listeria

46
Q

what ph level is tolerated by listeria

A

5.5 to 9.6

47
Q

motile at 25C with tumbling movement

A

listeria

48
Q

what causes meningoencephalitis in sheep

A

listerai innocua

49
Q

has the ability to invade both phagocytic and non-phagocytic cells

A

l. monocytogenes

50
Q

Able to replicate intracellularly and to transfer from cell to cell without exposure to humoral defense mechanisms

A

listeria

51
Q

small transparent colonies with smooth borders appear on blood agar in 24 hrs, becoming
greyish white and 0.5-2.0 mm in diameter in 48 hrs.

A

colonial appearance of listeria

52
Q

produces wide zone of hemolysis

A

l. ivanovii

53
Q

produces narrow zone of hemolysis

A

l. monocytogenes

54
Q

(aggregartion of leukocytes
on the perivascular space)

A

perivascular cuffing

55
Q

isolation of listeria

A

ox or sheep agar
macconkey
selective media

56
Q

in l. monocytogenes, how do we observe its tumbling motility

A

hanging drop ethod

57
Q

–umbrella-shaped, sub surface growth in semi- solid motility medium

A

listeria spp

58
Q

Intraperitoneal inoculation of listeris to mice

A

death within 5 days with necrotic lesions in the liver

59
Q

prevention and control of listeria

A

active immunization with viable l. mono
use of good quality low ph silage

60
Q

serious invasive disease that primarily afflicts pregnant women, neonates and immunocompromised adults.

A

listeriosis

61
Q

The causative organism, Listeria monocytogenes, is primarily transmitted to humans through

A

contaminated foods

62
Q

smooth form of erysipelothrix rhusiopathiae

A

gram pos slender rods

63
Q

up to 50% of pigs are carriers of what

A

erysipelothrix rhusiopathiae

64
Q

coagulase positive and h2s isproduced

A

erysipelothrix rhusiopatjiae

65
Q

causes septicemia, diamond lesions, chronic arthritis, chronic valvular endocarditis in pigs

A

erysipelothrix rhusiopathiae

66
Q

-widespread in the environment with proliferation in organic matter

A

erysipelothrix rhusiopatjiae

67
Q

-animal and bird, slime layer of fish, commonly associated with pigs (soil and slurry of piggeries), feces and tonsils
of carrier pigs

A

erysipelothrix rhusiopathiae

68
Q

virulence factors of erysipelothrix rhusiopathiae

A

neuraminidases
hyaluronidase
inflammatory cytokines
capsules

69
Q

acute form of disease of erysipelothrix rhusiopathiae

A

speticemic and cutaenous form

70
Q

chronic form of disease in ery

A

arthritis and vegetative endocarditis

71
Q

raised ,rhomboid, reddish ,purpluish lesion (diamond-shaped lesions are pathognomonic) encircled in red

A

swine erysipelas

72
Q

transmission of ery

A

ingestion of contaminated material, wound infections, and arthropod bites

73
Q

localized cellulitis)

A

erysipeloid

74
Q

skin ulcers, painful, erythematous,
but not purulent (encircled in green)

A

erysipeloid

75
Q

what bacteria is zoonotoc

A

erys`

76
Q

specimens in erys

A

liver kideny spleen heart and synovial fluid

77
Q

what morph of erys is seen in acute cases

A

rodsh

78
Q

what morph of erys is seen in chronic cases

A

filamentous

79
Q

isolation of erys

A

sheep of blood agar

80
Q

medium used for isolation of erys

A

selective medium that contains sodium azide, and crysrtal violet (Natellurite)

81
Q

what to observe in erys in Nutrient agar at room temp for 5 days

A

bottle brush pattern of growth

82
Q

prevention an dcontrol of erys

A

> proper disposal of infected carcass
isolate and introduce replacement animals into the herd at least 30 days into the herd
good sanitation and nutrition

83
Q

treatment of erys

A

penicillin, tetracycline, tylosin

84
Q
A

bacteriology (8 decks)

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