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bacteriology > myco > Flashcards

myco Flashcards

1
Q

this group of mycobacterium is Slow grower species-require number of weeks to produce observable colonies

A

tuberculosis group

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2
Q

species under the tuberculosis group of myco

A

m. tuberculosis, microti, africanum

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3
Q

the moree rapidly growing species of myco which is -atypical,anonymous,or nontubercular mycobacteria

A

runyons group

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4
Q

how many days will the runyon group produce colonies

A

3-21 days

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5
Q

mycobacteria included in mycobacterium complex

A

m. avium and intracellulare

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6
Q

the environmental and free living myco bacteria were placed in runyon in what year

A

1959

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7
Q

categories of runyon group were based on

A

rate of growth, colony morphology, pigment production

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8
Q

-producing pigmented (yellow colonies) only after exposure to light;slow growing;requires 7
days or more for visible colony

A

photochromogenic froup 1

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9
Q

producing yellow or orange pigment in the absence of light, slow growin

A
  1. scotochromogenic
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10
Q

-producing no or slight pigment with exposure to light; slow growing

A

nonphotochromogenic

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11
Q

grow rapidly with visible growth in less than 7 day

A

variable pigmentation

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12
Q

-inhibit phagosome-lysosome fusion within macrophage

A

sulfolipids

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12
Q

virulence factor of mycobacterium

A

mycosides
glycolipids
mycolic acid
sulfolipids

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13
Q

enhanced survival of phagocytosed mycobacteria; act to
scavenge and detoxify the toxigenic oxygen radicals that are produced by the macrophage oxidative burst

A

glycolipids

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13
Q

toxic, contribute to granulomatous response;

A

glycolipids

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14
Q

control of cellular permeability

A

mycosides

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15
Q

associated with cord factor that inactivates mitochondrial membranes within phagocyte and also inhibit
chemotaxis;

A

mycosides

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16
Q

also associated with wax D and various proteins that induce delayed –type hypersensitivit

A

mycosides

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16
Q

excreted in respiratory discharges, feces, milk, urine and semen

A

myco bovis

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17
Q

pathogenesis of m. bovis

A

Infection via respiratory and intestinal tracts→local multiplication extracellular and intracellular due to resistance to
phagocytic killings→infected host cell and bacteria reach local lymph nodes→thoracic duct→general
dissemination→macrophage activation kills some bacteria→macrophage aggregation contribute to tubercle formation
which may be encompass by fibrous layer→ lymphatic spread is retarded but continuous extension occurs via erosion of
bronchi, blood vessels or viscera to new area

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18
Q

specimen from live animal in myco

A

aspirates from cavities, lymph nodes, biopsies, tracheobronchial lavages and the
centrifuged deposit from 50 ml milk from suspected mastitis case

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19
Q

specimens of myco from dead animals

A

fresh and fixed samples of lesions or a selection of lymph nodes from a tuberculin reactor
with no visible lesions

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19
Q

can also be stained with fluorescent dye and viewed under UV microscop

A

myco

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20
Q

zn pos, slender, often beaded roots

A

m. bovis

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20
Q

Media for the mycobacterium

A

egg-based lowenstein-jensen and stonebrinks

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20
Q

isolation m. bovis

A

Selective decontamination to reduce fast-growing mycobacteria
Digestion or liquefaction of mucus
Centrifugation of samples to concentrate number of bacteria before smear preparation and culture

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21
Q

acts as prophylaxis in zoos and animal parks (myco)

A

isoniazid

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22
Q

vaccine for prevention of infestation of m. bovis

A

bgc (bacilli-calmette guerin

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22
Q

Treatment, Prevention and control: myco

A

isoniazid
bgs
early detection

22
Q

Tuberculin Tests commonly used:

A

tuberculin-purified protein derivative

23
Q

most widely used tuberculin test

A

intradermal

24
Q

inoculation sites of intradermal in tuberculin testing

A

caudal folds, vulvar lips, side of neck

24
Q

: intradermal inoculation of regular and avian tuberculin at two different sites in the neck. Read at
72 hours by measuring swelling

A

comparative cervical

24
Q

mostly used in primates; dilution of regular ‘bovine’ tuberculin is inoculated intradermally into the upper eyelid

A

opthalmis

24
Q

Pathogenesis and Pathogenicity Mycobacterium avium subsp. paratuberculosi

A

ingestion of feces, contaminated food and water
ingested bacteria is taken up by macrophage to the intestinal lymph node
immune mediated granulomatous reaction on the submucosa with accumulation of large numbers of macropgahe and lymphocytes
slough off submucosa
chronic enteritis and diarrhea
emaciation and death

25
Q

m. avium specimen to be collected from live animals

A

a small pinch biopsy from rectum or rectal scraping, biopsy from the mesenteric lymph nodes, fecal
samples

26
Q

m. avium specimen to be collected from dead animals

A

fresh and fixed samples of a section of the ileocecal valve, mesenteric lymph nodes

26
Q

in direct microscopy of m. avium, Smears of fecal and ileocecal valve mucosa stained with ZN reveals ___ which indicates intracellular growth

A

arge numbers of short, red staining
rods in clumps,

26
Q

m avium intravenous johnin test positive reaction

A

a rise in temp to 1.5 C or more

26
Q

serology testing for avium

A

Complement Fixation test for screening, Immunodiffusion, ELISA

26
Q

avium subsp. paratuberculosis has been isolated from tissues of several human patients with

A

crohn’s disease

26
Q

growth rate of m. tuberculosis and m. bovis

A

3-8 weeks

27
Q

growth rate of m. avium

A

2-6 weeks

27
Q

growth rate of m. avium subsp. paratuberculosis

A

ver slow upto 16 weeks

27
Q

optimal incubation of myco

A

37

27
Q

atmospheric requirement of myco

A

aerobic

27
Q

colonial features of m. tuberculosis

A

rough, buff, difficult to break apart

28
Q

colonial features of m. bovis

A

cream colored, raised with central rougness, break apart easily

29
Q

colonial features of m. avium

A

sticky off white, break off easily

30
Q

colonial features of m. avium subsp paratuberculosis

A

small, hemisherical, some pigmented

31
Q

essential growth supplemet of m. avium subsp paratuberculosis

A

mycobactin

32
Q

effect of added sodium pyruvate in myco

A

no effect except in bovis which is enhanced growth

33
Q

effect of added glycerol in myco

A

tuberculosis and avium- eugonic
bovis- dysgonic

34
Q

Formation of single or multiple granulomas or nodules of the skin 1-3 cm in diameter

A

mycobacterium lepraemurium

35
Q

Painless and move freely. May ulcerate
and discharge a slight, serosanguineus exudate. It is though that cats acquire infection via rat bites.

A

mycobacterium lepraemurium

36
Q

causes food poisoning to humans

A

bacillus cersus

37
Q

causes sporadic abortion in cattle and sheep

A

bacillus licheniformis

38
Q
A
39
Q

chromosomally coded virulence factors of bacillus anthracis

A

1.Extracellular proteases
2.Siderophores
3.Anthrolysin O
4.Manganes Ion ATP binding cassette
5. Phospholipase C

40
Q

pathogenicity of anthrax

A

Uptake of the spores by the host from the environment→ germination within the host and multiplication of the vegetative
form to large numbers→ bacteremia and toxemia→ death→ shedding of bacilli in the environment→sporulation.

41
Q

Diseased animals due to anthrax may suffer from:

A

> sudden, unexpected death
mild anorexia or reduced milk production in case of cattle
steep rise in temperature
exudation of tarry blood from body orifices, failure of blood to clot, incomplete rigor mortis, splenomegaly in cattle from
orifices at death

42
Q

how to kill spores of b. anthracis

A

autoclaving 121C/15 minutes amd dry heat 1500/60min. (not by boiling for under 10min)

43
Q

what type of disinfectants is useful for anthrax

A

aldehydes, oxidizing and chlorinating disinfectants, beta propiolactone, ethylene oxide

44
Q

what specie is highly resistant to anthrax

A

birds followedf by pigs and carnivores, then horses and humas

45
Q

most susceptible to anthrax

A

cattle, sheep goat

46
Q

sample specimen for anthrax

A

blood from ear or tal veins, edematous fluid, peritoneal fluid

47
Q

specimen for culture of anthracid

A

blood or homogenous spleen

48
Q

edematous swelling that is hot and painful later becomes old and painless

A

malognat carbuncle

49
Q

respiratory infections smong workers working with hides, woll and hair contaminated with spores

A

woolsorter’s disease

50
Q

bacilli is susceptible to

A

penicillin, streptomycin, tetracycline, fluoroquinolones, and erythromycin

51
Q

what specie of bacili is motile, resistant to penicillin, hemolytic,

A

cereus

52
Q
A

bacteriology (8 decks)

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