POM MOCK 9 - research, haematology pracs, fluid, cell signaling, Flashcards
Difference between normal range and reference range?
“Normal range” is a range derived from a healthy reference population. Reference ranges take into account age, gender, ethnic origin and physiological status (pregnant or not).
What guides data analysis?
The research question the data is trying to answer and the hypotheses to be tested.
What contributes to validity of dataset?
A standard operating procedure (SOP) for data collection and analysis
Small proportion of missing data
Explicit inclusion and exclusion criteria
A large and diverse sample
When can you exclude outliers?
A case of a mistake in data collection. e.g value inputted is out of range of acceptable values.
What are the two gene clusters that code for the haemoglobin molecule?
Alpha and beta cluster.
What is the P50 value?
Is the pressure at which hemoglobin is 50% saturated.
Explain the shape of Oxygen–haemoglobin dissociation curve?
Sigmoid. Once one haem group binds to oxygen it is easier for other groups to bind to oxygen. This is known as positive cooperative effect.
What does 2,3 dpg do to haemoglobin?
Binds to site in deoxyhaemoglobin distant from haem groups and reduces affinity for oxygen.
How is 2,3 dpg made?
Conversion of 1,3 bpg made in glycolysis to 2,3 bpg via Rapoport- Luebering shuttle.
Why does haemoglobin F have a greater affinity that haemoglobin A?
Single amino acid substitution in gamma chain of haemoglobin F results in reduced affinity for 2,3 bpg and so increased affinity for oxygen.
Why is it important that fetal haemoglobin has a greater affinity for oxygen than haemoglobin A?
Allows for O2 to flow from maternal oxyhaemoglobin to fetal deoxyhaemoglobin.
What is the bohr effect?
H+ and CO2 production facilitates oxygen release from oxyhaemoglobin. This can be seen in exercise.
In relation to the effect on haemoglobin what are H+ ions, CO2 and 2,3dpg?
Allosteric effectors.
What are different human haemoglobin type separation based on in electrophoresis?
Charge.
What is the beer lambert law used for?
To calculate concentration of substance using absorbance value.