Polyomaviridae

Question 1

What type of genome doe polyomavirus have?

Answer 1

circular dsDNA

non-enveloped

Question 2

Where does replication of polyomavirus take place?

Answer 2

Nucleus

Question 3

T/F

Papillomavirus can be cultured in vitro

Answer 3

False

Polyomavirus can

Question 4

What disease does polyomavirus cause in birds?

Answer 4

Budgrigar fledgling polyomavirus

Question 5

What age group does Budgrigar fledgling polyomavirus affect?

Answer 5

Young

Question 6

Is BFDV fatal?

Answer 6

Yes

Question 7

What does BFDV present like?

Answer 7

Abnormal feathering (>15 days old)
Ascites (<15 days old)
Sudden, unexpected death after brief weakness

Question 8

How is BFDV transmitted?

Answer 8

Horizontal spread via feces, urine, respiratory secretions, and feather dust

Vertical spread – egg transmission

Question 9

What birds provide source of infection?

Answer 9

birds that present as normal asymptomatic carriers

Question 10

What is the hallmark histophatologic appearance for BFDV? Best tissue to sample?

Answer 10

karyomegaly w/ marginated chromation and large basophilic - clear intranuclear inclusions

liver and spleen

Question 11

How do you diagnose BFDV?

Answer 11

Clinical signs, Hx, and histologic lesion

PCR – test cloacal swabs

Question 12

Is there a polyomavirus vaccine?

Answer 12

Yes

Question 13

What does a negative polyomavirus PCR mean to us?

Answer 13

Not necessarily negative but DNA just wasn’t detected.

We only trust a positive result

Question 14

T/F

Polyomavirus positive birds must be reported and disposed of

Answer 14

False

they can be kept as pets if kept away from other birds