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CLINICAL CHEMISTRY 2 > PHOSPHATASES > Flashcards

PHOSPHATASES Flashcards

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1
Q

from what class is alkaline phosphatase

A

hydrolases

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2
Q

systematic name of alp

A

Orthophosphoric Monoester
Phosphohydrolase (Alkaline
Optimum)

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3
Q

product of alp

A

alcohol
phosphate ion

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3
Q

optimum pH of alp

A

9.0 -10.0

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4
Q

activators of alp

A

magnesium
zinc

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4
Q

substrate of bessey-lowry-brock method

A

p-nitrophenylphosphate

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5
Q

p-nitrophenyl phosphate (colorless) is HYDROLYZED to

A

p-nitrophenol (yellow)

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5
Q

Calculation of ALP activity based on the molar absorptivity of p-nitrophenol

A

BOWERS AND MCCOMB

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6
Q

CONTINUOUS-MONITORING TECHNIQUE is …proportional to alp activity

A

DIRECTLY PROPORTIONAL

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6
Q

tissue sources of alp

A

liver
bone
kidney
spleen
intestine
placenta

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6
Q

absorbance of alp

A

405 nm

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6
Q

major tissue source of acp

A

prostate

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7
Q

most anodal furthest to cathode

A

liver alp

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8
Q

most heat labile

A

bone alp

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9
Q

least heat labile

A

placenta alp

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10
Q

least anodal nearest to cathode

A

intestinal alp

10
Q

isoenzymes of acp

A

prostatic
lysosomal
macrophage
erythrocytic
osteoclastic

11
Q

elevated during adenocarcinoma of the pancreas

A

nagao alp

12
Q

most heat stable alp (in general)

A

regan alp

13
Q

abundant in cancers affecting the lungs, breasts, and gynecological areas

A

regan alp

14
Q

important in differentiating bone alp and regan alp

A

phenylalanine reagent

15
Q

major source of acp

A

prostate

16
Q

what sample that men produce where acp is found

A

semen

17
Q

most common method of measuring alp kinetic method

A

bowers and mccomb

18
Q

substrate of bowers and mccomb

A

para-nitrophenylphosphate

19
Q

ph of P-npp

A

10.2

20
Q

products of para-nitrophenylphosphate

A

phosphate ion
para-nitrophenol

21
Q

what color is para-nitrophenol after is changed its absorbance

A

yellow

22
Q

inactivates alp

A

phosphorus

23
Q

prevents the inactivation of alp

A

2-amino-2-methyl-1-propanol

24
Q

result of hemolysis in acp

A

falsely increase

25
Q

inhibits intestinal and placental alp to a much greater extent than liver and bone alp

A

phenylalanine

26
Q

substrate of choice for quantitative endpoint reactions

A

thymolphthalein monophosphate

27
Q

substrate choice for continuous monitoring methods

A

alpha-napththyl phosphate

28
Q

inhibit phosphorus present in serum + mg/zinc in serum

A

2-amino-2-methyl-propanolol

29
Q

blood type that binds to erythrocytes

A

type a

30
Q

carcinoplacental isoenzymes

A

regan
nagao
kasahara

31
Q

substrate of acp

A

phosphomonoesters

32
Q

assay that does not need of serum to acidify

A

radioimmunoassay

CLINICAL CHEMISTRY 2 (9 decks)

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