PHOSPHATASES Flashcards
trans base
from what class is alkaline phosphatase
hydrolases
systematic name of alp
Orthophosphoric Monoester
Phosphohydrolase (Alkaline
Optimum)
product of alp
alcohol
phosphate ion
optimum pH of alp
9.0 -10.0
activators of alp
magnesium
zinc
substrate of bessey-lowry-brock method
p-nitrophenylphosphate
p-nitrophenyl phosphate (colorless) is HYDROLYZED to
p-nitrophenol (yellow)
Calculation of ALP activity based on the molar absorptivity of p-nitrophenol
BOWERS AND MCCOMB
CONTINUOUS-MONITORING TECHNIQUE is …proportional to alp activity
DIRECTLY PROPORTIONAL
tissue sources of alp
liver
bone
kidney
spleen
intestine
placenta
absorbance of alp
405 nm
major tissue source of acp
prostate
most anodal furthest to cathode
liver alp
most heat labile
bone alp
least heat labile
placenta alp
least anodal nearest to cathode
intestinal alp
isoenzymes of acp
prostatic
lysosomal
macrophage
erythrocytic
osteoclastic
elevated during adenocarcinoma of the pancreas
nagao alp
most heat stable alp (in general)
regan alp
abundant in cancers affecting the lungs, breasts, and gynecological areas
regan alp
important in differentiating bone alp and regan alp
phenylalanine reagent
major source of acp
prostate
what sample that men produce where acp is found
semen
most common method of measuring alp kinetic method
bowers and mccomb
substrate of bowers and mccomb
para-nitrophenylphosphate
ph of P-npp
10.2
products of para-nitrophenylphosphate
phosphate ion
para-nitrophenol
what color is para-nitrophenol after is changed its absorbance
yellow
inactivates alp
phosphorus
prevents the inactivation of alp
2-amino-2-methyl-1-propanol
result of hemolysis in acp
falsely increase
inhibits intestinal and placental alp to a much greater extent than liver and bone alp
phenylalanine
substrate of choice for quantitative endpoint reactions
thymolphthalein monophosphate
substrate choice for continuous monitoring methods
alpha-napththyl phosphate
inhibit phosphorus present in serum + mg/zinc in serum
2-amino-2-methyl-propanolol
blood type that binds to erythrocytes
type a
carcinoplacental isoenzymes
regan
nagao
kasahara
substrate of acp
phosphomonoesters
assay that does not need of serum to acidify
radioimmunoassay
