CC2 LAB: CREATININE KINASE, ALT, AST Flashcards
ppt based
1
Q
associated with atp regeneration in contractile or transport system
A
creatinine kinase
2
Q
ck is predominant in
A
muscle cells
3
Q
how many kDa in ck
A
82
4
Q
separation of atypical bands
detection of ak
A
electrophoresis (reference method)
5
Q
good with technique, may be more sensitive and precise
A
ion-exchange chromatography
6
Q
measure concentration rather than activity
may detect inactive ck-mb
A
radioimmunoassay
7
Q
- anti-M inhibits M activity
- CK activity is measure before and after inhibition
- residual activity is multiplied by 2 to account for MB
(50% inhibited) - disadvantage: detects BB activity; though small amount,
may cause false MB activity; also, atypical forms are not
inhibited
A
immunoinhibition
8
Q
A in Coupled-enzyme method
A
340 nm
9
Q
forward reaction in ck
A
Tanzer-Gilvarg
10
Q
- reverse reaction in ck
- most common
- 2 – 6x faster than forward
- less interference
A
Oliver Rosalki
11
Q
Tanzer-Gilvarg Method pH
A
pH 9.0
12
Q
Oliver-Rosalki Method pH
A
pH 6.8
13
Q
inactivated because of oxidation of sulfhydryl groups
A
CK activity unstable
14
Q
T/F: Hemolyzed samples falsely elevate CK activity
A
T
15
Q
ratio of ck
A
1:4
