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CLINICAL CHEMISTRY 2 > CC2 LAB: LACTATE DEHYDROGENASE AND CREATININE KINASE > Flashcards

CC2 LAB: LACTATE DEHYDROGENASE AND CREATININE KINASE Flashcards

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1
Q

catalyzes the interconversion of lactic and pyruvic acid

A

lactate dehydrogenase

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2
Q

from what major class is lactate dehydrogenase

A

oxidoreductase

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3
Q

how many daltons does ld have

A

128 kDa

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4
Q

how many subunits are there in lactate dehydrogenase

A

4 (tetrameric)

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5
Q

arrangement of isoenzymes

A

LDH1
LDH2
LDH3
LDH4
LDH5

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6
Q

abnormal isoenzyme

A

LDH6

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7
Q

tissue source for LDH1 to LDH5

A

LDH1&2: heart, rbc
LDH3: lung, lymohocyte, spleen, pancreas
LDH4: liver
LDH5: skeletal muscle

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8
Q

related to heart diseases

A

LDH1

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9
Q

related to hepatic disorders

A

LDH5

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10
Q

most ubiquitous out of all the LDH units

A

LDH3

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11
Q

serum row of a normal person

A

LDH2>LDH1>LDH3>LDH4>LDH5

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12
Q

heart row of a normal person

A

LDH1>LDH2>LDH3>LDH4>LDH5

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13
Q

substrate of lactate dehydrogenase

A

alpha-hydroxybutyrate

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14
Q

in electrophoresis the complex is made up of

A

IgA
IgG

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15
Q

what do you call the forward method and what is its optimal pH

A

Wackers method: 8.3-8.9pH

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16
Q

what do you call the backward method and what is its optimal pH

A

wroblewski la due method: 7.1-7.9pH

16
Q

LD should be stored at RT within

A

48hrs

16
Q

most labile LD

A

LD-5

16
Q

associated with atp regeneration in contractile or transport system

A

creatinine kinase

16
Q

LD isoenzyme analysis should be stored at RT and analyzed within

A

24hrs of collection

17
Q

LDH changes after how many days of storage of serum at rt

A

3 days

17
Q

from what major class is creatinine kinase

A

transferases

17
Q

ck3

A

cardiac and skeletal muscles

18
Q

ck1

A

brain

18
Q

ck2

A

cardiac muscles

19
Q

fastest to migrate toward anode

A

ck1

20
Q

slowest mobility

A

ck3

21
Q

major isoenzyme in the sera of healthy people that is located in the striated muscles

A

ck3

22
Q

present in small quantities in the sera of healthy people

A

ck1

23
Q

undetectable to trace in healthy people

A

ck2

24
Q

detection of AK in hemolyzed samples

A

electrophoresis

25
Q

migrate to a position midway between ck-mb and ck-mm

A

adenylate kinase

26
Q

migrate to a point cathodal to ck-mm

A

mitochondrial-ck

27
Q

exhange of ions between columns or is separated into columns

A

ion-exchange chromatography

28
Q

coupled-enzyme activity is a change in A at what absorbance

A

340 nm

29
Q

measure concentration rather than activity

A

radioimmunoassay

30
Q

forward reaction in cea and what pH

A

tanzer-gilvarg method
9.0pH

31
Q

reverse reaction in cea and what pH

A

oliver rosalki method
6.8pH

CLINICAL CHEMISTRY 2 (9 decks)

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