Phlebotomy

Question 1

On which finger will you perform Capillary Blood Collection?

Answer 1

3rd (middle) or 4th (ring) finger

Question 2

In Ivy’s/Template Method, until when will you inflate the blood pressure cuff?

Answer 2

40 mmHg (Adults)

20 mmHg (Toddlers)

Question 3

Every how many seconds will you blot blood from the incision (bleeding time)?

Answer 3

every 30 seconds

Question 4

What site is used for Ivy’s/Template Method?

Answer 4

Volar Surface of the forearm

Question 5

What site is used for Duke’s Method (Bleeding time)?

Answer 5

Fingertip (preferably the ring finger)

Question 6

Two methods used in Clotting time

Answer 6

1. Lee and White Method
2. Slide Method

Question 7

How many tubes of blood will you use for Lee and White and how much (in mL) do you need?

Answer 7

Three red top tubes

at least 1 mL

Question 8

Time starts when? (Lee and White Method)

Answer 8

When the 3rd tube is filled with blood

Question 9

Tilt the tubes every __ minute to observe for clot

Answer 9

1

Question 10

procedure stops when there is _______ ________ formed in the three tubes. (Lee and White method)

Answer 10

visible clotting

Question 11

Tests stop when a ______ _______ _______ is observed. (Slide Method)

Answer 11

Visible fibrin strand

Question 12

Angle when preparing a Peripheral Blood Smear

Answer 12

35-45 degrees

Question 13

Angle for Malarial Smear

Answer 13

45 degrees

Question 14

Number of inversions:

Blood Cultures - SPS

Answer 14

8-10 times

Question 15

Number of inversions:

Citrate tube

Answer 15

3-4 times

Question 16

Number of inversions:

BD Vacutainer SST Gel Separator Tube

Answer 16

5 times

Question 17

Number of inversions:

Serum tube (glass or plastic)

Answer 17

plastic: 5 times

Glass: none

Question 18

Number of inversions:

BD Vacutainer Rapid Serum Tube

Answer 18

5 to 6 times

Question 19

BD Vacutainer PST Gel Separator Tube with Heparin

Answer 19

8-10 times

Question 20

Number of inversions:

Heparin tube

Answer 20

8-10 times

Question 21

Number of inversions:

EDTA tube

Answer 21

8 to 10 times

Question 22

Number of inversions:

BD Vacutainer PPT Separator Tube K2EDTA with Gel

Answer 22

8 to 10 times

Question 23

Number of inversions:

Fluoride (glucose) tube

Answer 23

8 to 10 times

Question 24

Additive in Blood Culture Bottles

Answer 24

SPS (Sodium Polyanethol Sulfonate) or ACD

Question 25

Additive in Light blue tube

Answer 25

3.2% Sodium Citrate

Question 26

Additive in Red or Plain top

Answer 26

no additive

Question 27

Additive in Serum Separating Tube (SST)

Answer 27

Serum Separating Gel and clot activator

Question 28

Additive in Green top

Answer 28

Sodium Heparin or Lithium Heparin

Question 29

Additive in Purple or Lavender top

Answer 29

EDTA

Question 30

Additive in Gray top

Answer 30

sodium fluoride (glycolysis inhibitor)

potassium oxalate (anticoagulant)