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Comprehensive Exam > Cellular Immunology > Flashcards

Cellular Immunology Flashcards

1
Q

field concerning the interactions among cells and molecules of the immune system, and how such interactions contribute to the recognition and elimination of pathogens

A

Cellular Immunology

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2
Q

passage of cells in single file in front of a laser so they can be detected, counted and sorted.

A

flow cytometry

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3
Q

forward scatter

A

cell size

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4
Q

side scatter

A

cell granularity/lobes

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5
Q

Tests with Flow Cytometry as Principle

A
  1. FACS CD4
  2. Primary Immunodeficiency Panel 1
  3. Primary Immunodeficiency Panel 3
  4. Flow PRA
  5. Flow Cytometric Crossmatching
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6
Q

used to enumerate absolute counts of CD4 T lymphocytes

A

FACS CD4

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7
Q

aka lymphocyte subset enumeration test

A

PID 1 and PID 3

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8
Q

CD is an abbreviation for

A

cluster of differentiation

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9
Q

CD45

A

backbone marker

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10
Q

CD3

A

Pan T lymphocytes

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11
Q

CD4

A

T-helper cells

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12
Q

CD8

A

T Suppressor cells/Cytotoxic T cells

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13
Q

CD16/56

A

Natural Killer Cells

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14
Q

CD19

A

Pre B cells

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15
Q

CD20

A

Pan B cells

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16
Q

routine screening measure to assess the degree of a potential kidney recipient’s sensitization, as a result of prior exposure to external HLA antigens during previous blood transfusion, pregnancies, or organ transplantations

A

Flow PRA

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17
Q

aka T and B cell crossmatching

A

Flow Cytometric Crossmatching

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18
Q

What marker is aka Decay Accelerating Factor?

A

CD55

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19
Q

What marker is aka Membrane Inhibitor of Reactive Lysis?

A

CD59

20
Q

characterized by destruction of RBCs, blood clots, and impaired bone marrow function

A

Paroxysmal Nocturnal Hemoglobinuria

21
Q

aka Ham Test

A

PNH Panel

22
Q

aka T cell Crossmatching

A

Tissue crossmatching

23
Q

diseases associated with HLA B27

A

Ankylosing Spondylitis
Reactive Arthritis
Uveitis

24
Q

aka MHC (Major Histocompatibility Complex)

A

HLA (Human Leukocyte Antigen)

25
Q

proteins that help the body’s immune to tell difference between self and non self

A

HLA

26
Q

a procedure in which the tissues of a prospective donor and recipient are tested for compatibility prior to transplantation. Mismatched donor and recipient tissues can kead ro rejection of the tissues; test used to match patients and donors for bone marrow, cord blood, or organ transplants

A

HLA Typing

27
Q

Machine used for HLA Tissue typing Class I and II

A

ABI 7500 Fast Real Time PCR

28
Q

Machine used for HLA DNA Typing with C and DP

A

LUMINEX LABScan 100

29
Q

aka Human Polyomavirus 1 that causes trivial symptoms in the immunocompetent, although it may cause significant pathology in the immunosuppressed transplant recipient

A

BK virus

30
Q

Machine used for COVID-19

A

Biorad Cfx 96 Real Time System

31
Q

Sansure COVID Real Time Assays undergo ___ cycles of amplification

A

45

32
Q

References center for Newborn Screening

A

UP - National Institute of Health (NIH)

33
Q

Newborn Screening Act of 2004

A

Republic Act No. 9288

34
Q

defined as the number of cycles required for the fluorescent signal to cross the threshold

A

CT Values (Cycle Threshold)

35
Q

Principle of STR DNA Anallysis and STR Engraftment Analysis/Chimerism

A

Capillary Electropheresis

36
Q

to evaluate if the chromosome 1q or 19q has deletion or no deletion

A

FISH 1P19Q

37
Q

specimen for FISH1P19Q

A

tissue block (brain)

38
Q

specimens for BK Virus

A

Plasma/Serum/Urine

39
Q

Principle of T-Spot TB test

A

IGRA (Interferon Gamma Release Assay)

40
Q

Princile of T-Spot TB test

A

IGRA (Interferon Gamma Release Assay)

41
Q

What machine is used to perform Human Papilloma Virus (HPV) High Risk with 16, 18/45 Genotyping

A

Cepheid GeneXpert Instrument Systems

42
Q

Machine used for:

STR DNA Analysis (Short Tandem Repeat)
STR Engraftment Analysis/Chimerism

A

ABI 3500 Genetic Analyzer

43
Q

Machine used for COVID-19

A

Nextractor

44
Q

Sample requirement for HBV/HCV/HIV Viral load

A

Plasma

45
Q

CD34 is a marker for

A

Blasts

Comprehensive Exam (9 decks)

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