Cellular Immunology Flashcards
field concerning the interactions among cells and molecules of the immune system, and how such interactions contribute to the recognition and elimination of pathogens
Cellular Immunology
passage of cells in single file in front of a laser so they can be detected, counted and sorted.
flow cytometry
forward scatter
cell size
side scatter
cell granularity/lobes
Tests with Flow Cytometry as Principle
- FACS CD4
- Primary Immunodeficiency Panel 1
- Primary Immunodeficiency Panel 3
- Flow PRA
- Flow Cytometric Crossmatching
used to enumerate absolute counts of CD4 T lymphocytes
FACS CD4
aka lymphocyte subset enumeration test
PID 1 and PID 3
CD is an abbreviation for
cluster of differentiation
CD45
backbone marker
CD3
Pan T lymphocytes
CD4
T-helper cells
CD8
T Suppressor cells/Cytotoxic T cells
CD16/56
Natural Killer Cells
CD19
Pre B cells
CD20
Pan B cells
routine screening measure to assess the degree of a potential kidney recipient’s sensitization, as a result of prior exposure to external HLA antigens during previous blood transfusion, pregnancies, or organ transplantations
Flow PRA
aka T and B cell crossmatching
Flow Cytometric Crossmatching
What marker is aka Decay Accelerating Factor?
CD55
What marker is aka Membrane Inhibitor of Reactive Lysis?
CD59
characterized by destruction of RBCs, blood clots, and impaired bone marrow function
Paroxysmal Nocturnal Hemoglobinuria
aka Ham Test
PNH Panel
aka T cell Crossmatching
Tissue crossmatching
diseases associated with HLA B27
Ankylosing Spondylitis
Reactive Arthritis
Uveitis
aka MHC (Major Histocompatibility Complex)
HLA (Human Leukocyte Antigen)
proteins that help the body’s immune to tell difference between self and non self
HLA
a procedure in which the tissues of a prospective donor and recipient are tested for compatibility prior to transplantation. Mismatched donor and recipient tissues can kead ro rejection of the tissues; test used to match patients and donors for bone marrow, cord blood, or organ transplants
HLA Typing
Machine used for HLA Tissue typing Class I and II
ABI 7500 Fast Real Time PCR
Machine used for HLA DNA Typing with C and DP
LUMINEX LABScan 100
aka Human Polyomavirus 1 that causes trivial symptoms in the immunocompetent, although it may cause significant pathology in the immunosuppressed transplant recipient
BK virus
Machine used for COVID-19
Biorad Cfx 96 Real Time System
Sansure COVID Real Time Assays undergo ___ cycles of amplification
45
References center for Newborn Screening
UP - National Institute of Health (NIH)
Newborn Screening Act of 2004
Republic Act No. 9288
defined as the number of cycles required for the fluorescent signal to cross the threshold
CT Values (Cycle Threshold)
Principle of STR DNA Anallysis and STR Engraftment Analysis/Chimerism
Capillary Electropheresis
to evaluate if the chromosome 1q or 19q has deletion or no deletion
FISH 1P19Q
specimen for FISH1P19Q
tissue block (brain)
specimens for BK Virus
Plasma/Serum/Urine
Principle of T-Spot TB test
IGRA (Interferon Gamma Release Assay)
Princile of T-Spot TB test
IGRA (Interferon Gamma Release Assay)
What machine is used to perform Human Papilloma Virus (HPV) High Risk with 16, 18/45 Genotyping
Cepheid GeneXpert Instrument Systems
Machine used for:
STR DNA Analysis (Short Tandem Repeat)
STR Engraftment Analysis/Chimerism
ABI 3500 Genetic Analyzer
Machine used for COVID-19
Nextractor
Sample requirement for HBV/HCV/HIV Viral load
Plasma
CD34 is a marker for
Blasts
