Cellular Immunology Flashcards

1
Q

field concerning the interactions among cells and molecules of the immune system, and how such interactions contribute to the recognition and elimination of pathogens

A

Cellular Immunology

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
2
Q

passage of cells in single file in front of a laser so they can be detected, counted and sorted.

A

flow cytometry

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
3
Q

forward scatter

A

cell size

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
4
Q

side scatter

A

cell granularity/lobes

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
5
Q

Tests with Flow Cytometry as Principle

A
  1. FACS CD4
  2. Primary Immunodeficiency Panel 1
  3. Primary Immunodeficiency Panel 3
  4. Flow PRA
  5. Flow Cytometric Crossmatching
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
6
Q

used to enumerate absolute counts of CD4 T lymphocytes

A

FACS CD4

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
7
Q

aka lymphocyte subset enumeration test

A

PID 1 and PID 3

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
8
Q

CD is an abbreviation for

A

cluster of differentiation

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
9
Q

CD45

A

backbone marker

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
10
Q

CD3

A

Pan T lymphocytes

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
11
Q

CD4

A

T-helper cells

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
12
Q

CD8

A

T Suppressor cells/Cytotoxic T cells

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
13
Q

CD16/56

A

Natural Killer Cells

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
14
Q

CD19

A

Pre B cells

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
15
Q

CD20

A

Pan B cells

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
16
Q

routine screening measure to assess the degree of a potential kidney recipient’s sensitization, as a result of prior exposure to external HLA antigens during previous blood transfusion, pregnancies, or organ transplantations

A

Flow PRA

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
17
Q

aka T and B cell crossmatching

A

Flow Cytometric Crossmatching

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
18
Q

What marker is aka Decay Accelerating Factor?

A

CD55

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
19
Q

What marker is aka Membrane Inhibitor of Reactive Lysis?

20
Q

characterized by destruction of RBCs, blood clots, and impaired bone marrow function

A

Paroxysmal Nocturnal Hemoglobinuria

21
Q

aka Ham Test

22
Q

aka T cell Crossmatching

A

Tissue crossmatching

23
Q

diseases associated with HLA B27

A

Ankylosing Spondylitis
Reactive Arthritis
Uveitis

24
Q

aka MHC (Major Histocompatibility Complex)

A

HLA (Human Leukocyte Antigen)

25
proteins that help the body's immune to tell difference between self and non self
HLA
26
a procedure in which the tissues of a prospective donor and recipient are tested for compatibility prior to transplantation. Mismatched donor and recipient tissues can kead ro rejection of the tissues; test used to match patients and donors for bone marrow, cord blood, or organ transplants
HLA Typing
27
Machine used for HLA Tissue typing Class I and II
ABI 7500 Fast Real Time PCR
28
Machine used for HLA DNA Typing with C and DP
LUMINEX LABScan 100
29
aka Human Polyomavirus 1 that causes trivial symptoms in the immunocompetent, although it may cause significant pathology in the immunosuppressed transplant recipient
BK virus
30
Machine used for COVID-19
Biorad Cfx 96 Real Time System
31
Sansure COVID Real Time Assays undergo ___ cycles of amplification
45
32
References center for Newborn Screening
UP - National Institute of Health (NIH)
33
Newborn Screening Act of 2004
Republic Act No. 9288
34
defined as the number of cycles required for the fluorescent signal to cross the threshold
CT Values (Cycle Threshold)
35
Principle of STR DNA Anallysis and STR Engraftment Analysis/Chimerism
Capillary Electropheresis
36
to evaluate if the chromosome 1q or 19q has deletion or no deletion
FISH 1P19Q
37
specimen for FISH1P19Q
tissue block (brain)
38
specimens for BK Virus
Plasma/Serum/Urine
39
Principle of T-Spot TB test
IGRA (Interferon Gamma Release Assay)
40
Princile of T-Spot TB test
IGRA (Interferon Gamma Release Assay)
41
What machine is used to perform Human Papilloma Virus (HPV) High Risk with 16, 18/45 Genotyping
Cepheid GeneXpert Instrument Systems
42
Machine used for: STR DNA Analysis (Short Tandem Repeat) STR Engraftment Analysis/Chimerism
ABI 3500 Genetic Analyzer
43
Machine used for COVID-19
Nextractor
44
Sample requirement for HBV/HCV/HIV Viral load
Plasma
45
CD34 is a marker for
Blasts