Hematology

Question 1

o Lilac/purple
o Thin/small granules
o 3-5 lobes
o No affinity to methylene blue and eosin
(NEUTRAL)

Answer 1

Neutrophil

Question 2

o Stains orange-pink
o Medium size granules
o Bi-lobed
o Has affinity to acid/eosin

Answer 2

Eosinophil

Question 3

o ↓ among all WBC
o Histamine granules
o Dark blue granules (methylene blue)
o 3 lobes
o Nucleus is not round

Answer 3

Basophil

Question 4

o Responsible for phagocytosis
o No nucleoli
o Small granules
o Vacuolations
o Irregular nucleus (S, horseshoe, kidney)
o Grayish blue cytoplasm
o BIGGEST CELL IN BLOOD

Found in viral infection

Answer 4

Monocytes

Question 5

o Irregular/oblong nucleus
o Has nucleoli
o Close to RBC
o increased in children

Answer 5

Lymphocytes

Question 6

principle of SYSMEX XN 3000

Answer 6

Main: Electrical Impedance with Hydrodynamic Focusing (for RBC and Platelets)

Differential Count (WBC): Fluorescence Flow Cytometry

Question 7

principle of BECKMAN COULTER DXH 900

Answer 7

Main: Electrical Impendance

Question 8

principle of DI (DIGITAL IMAGING) 60

Answer 8

ANN (Artificial Neural Network)

Digital Morphology (with built-in microscope-OLYMPUS)

Question 9

Automated Slide Reader

Answer 9

DI (DIGITAL IMAGING) 60

Question 10

Automated slide processor with stain preparation (15 minutes)

Answer 10

SP10

Question 11

principle of SYSMEX CS 2500-A (main machine for PT PTT)

Answer 11

Photo-Optical uses Transmitted Light Detection Method

Question 12

principle of SYSMEX CA 101

Answer 12

Opto-Mechanical via Turbodensitometric Method

Question 13

Back-up machine for PT/PTT

Answer 13

SYSMEX CS 2500-B

Question 14

principle of MULTIPLATE ANALYZER

Answer 14

Impedance Aggregometry

Question 15

tests done in MULTIPLATE ANALYZER

Answer 15

Clopidogrel Response Monitoring Test (ADP test)

Aspirin Response Monitoring Test (ASPItest)

Question 16

principle of ALCOR SCIENTIFIC iSED

Answer 16

Photometric Rheology

Question 17

Flaggings: WBC Left Shift

Answer 17

Immature Cells

Question 18

Flaggings WBC Right Shift

Answer 18

Hyper segmentation

Question 19

reference interval for Hemoglobin (female)

Answer 19

11.6-15.5

Question 20

stain commonly used for peripheral blood and bone marrow films

Answer 20

Wright and Wright-Giemsa stains

Question 21

termed polychrome stains (since they contain both eosin and methylene blue)

Answer 21

Wright or Wright Giemsa stain

Question 22

free methylene blue is?

Answer 22

basic and stains acidic cellular components, such as RNA, blue

Question 23

Free eosin is?

Answer 23

acidic and stains basic components such as Hemoglobin and eosinophilic granules, red,

Question 24

Giemsa stain

Answer 24

for platelets, RBC morphology, normal diff count, PBS

Question 25

Wright’s Giemsa Stain

Answer 25

for abnormal diff count (immature cells)

Question 26

engulfs and has enzymes that destroy bacteria and other pathogens; most abundant; plays an important role in fighting many types of bacterial infection

Answer 26

neutrophil

Question 27

normally increased in geriatric and pediatric patients

Answer 27

lymphocyte

Question 28

helps fight microorganisms and parasitic infections

Answer 28

eosinophil

Question 29

travels through the blood to tissues in the body where it become a macrophage or a dendritic cell.

Answer 29

Monocyte

Question 30

granules are filled with various proteins and chemicals like histamine, serotonin, and heparin

Answer 30

basophil

Question 31

increased RBC indices

Answer 31

cold agglutinin

Question 32

what does a perfect histogram look like?

Answer 32

bell shaped

Question 33

Reagent of PT

Answer 33

Thromborel S

Question 34

Reagent of PTT

Answer 34

Pathrombin SL, CaCl2

Question 35

Criteria for rejection of samples:

Answer 35

1. Hemolyzed
2. QNS (Qnty not sufficient)
3. Overfilled

Question 36

machine used for ESR

Answer 36

Alcor Scientific iSED

Question 37

machine used for ESR

Answer 37

Alcor Scientific iSED