Microbiology

Question 1

Steps in Gram Stain

Answer 1

1. heat fix for 5-10 secs
2. Crystal Violet
3. Gram’s Iodine
4. Acetone Alcohol
5. Safranin

Question 2

Steps in Acid Fast Stain

Answer 2

1. Carbol fuschin
2. Acid alcohol
3. Malachite green

Question 3

A prenatal test for GBS is used to detect the presence of this bacteria in the vagina and/or rectum of pregnant women. GBS can pass from pregnant woman to her fetus during labor. This
is rare and happens to 1 to 2 babies out of 100 when the mother does not receive treatment with antibiotics during labor.

Answer 3

Group ‘B’ Strep (GBS)
Screening Test

Question 4

Rapid immunochromatographic test for the qualitative detection of Influenza A and B virus antigens from Nasopharyngeal swab, throat swab, and nasal aspirate samples.

Answer 4

.
Influenza A & B Plus

Question 5

principle of Bact/Alert Virtuo for Blood Culture

Answer 5

Colorimetric

Question 6

Blood culture bottle containing a resin that removes
antimicrobials (antibiotics) from the specimen.

Answer 6

Antibiotic Removing Device (ARD)

Question 7

principle of Biofire Filmarray (TORCH)

Answer 7

Nested Multiplex PCR

Question 8

principle of Vitek MALDITOF-MS

Answer 8

Matrix Assisted Light Desorption Ionization – Time of
Flight Mass Spectrometry

Question 9

principle of GeneXpert

Answer 9

Reverse Transcription Polymerase Chain Reaction (RT-PCR)

Question 10

Hektoen Agar

Answer 10

Salmonella and Shigella

Question 11

Tellurite Blood Agar

Answer 11

C.diptheriae

Question 12

BUFFERED CHARCOAL YEAST AGAR

Answer 12

Legionella pneumophilia

Question 13

TCBS

Answer 13

Vibrio cholerae

Question 14

LOWENSTEIN-JENSEN AGAR

Answer 14

Mycobacterium tuberculosis

Question 15

MANNITOL SALT AGAR

Answer 15

S. aureus

Question 16

TSI / BAP

Answer 16

Corynebacterium

Question 17

CAP

Answer 17

Haemophilus

Question 18

MODIFIED THAYER MARTIN

Answer 18

Neisseria

Question 19

EMB

Answer 19

E.coli (Greenish Metallic Sheen)

Question 20

XLD

Answer 20

Enterobacteriaceae
(Salmonella and Shigella)

Question 21

BORDET GENGOU

Answer 21

Bordetella

Question 22

CYCLOSERINE CEFLOXIN FRUCTOSE
AGAR

Answer 22

Clostridium difficile

Question 23

FLETCHER MEDIUM

Answer 23

Leptospira spp.

Question 24

differential stain to classify bacteria into two major groups: Gram pos and Gram neg

Answer 24

Gram stain

Question 25

a laboratory test that determines if a sample or specimen is infected with the bacteria that causes Tuberculosis and other acid-fast org.

Answer 25

acid fast stain

Question 26

a prenatal test for GBS is used to detect the presence of this bacteria in the vagina and/or rectum of a pregnant woman.

Answer 26

Group B Strep (GBS) Screening Test

Question 27

exploits fluorescence of an oxygen sensor to detect growth of mycobacteria in culture

Answer 27

BACTEC MGIT 960