pgs. 161-166

Question 1

What is the patch-patch method?

Answer 1

candidate antibiotic producers are patched around a single edge of the plate, tester strain in middle, separated by a gap

Question 2

What is the spread-patch method?

Answer 2

tests for antibiotic production by candidate isolates that are in close physical contact with tester strain

Question 3

What is the top agar method?

Answer 3

liquefied agar is mixed with the tester strain and poured over patches of the soil isolates

Question 4

What does aerobic mean for liquid culture?

Answer 4

incubate with shaking

Question 5

What does aerobic mean for solid culture?

Answer 5

all strains should be grown in regular aerobic conditions

Question 6

How does E. carotovora grow?

Answer 6

grows fairly slowly in liquid culture

Question 7

How does L. antibioticus grow?

Answer 7

grows slowly on a plate

Question 8

How does S. epidermidis grow?

Answer 8

grows very slowly in the antibiotic assays

Question 9

What colony morphologies does A. baylyi have?

Answer 9

opaque and white-coloured