PERIPHERAL SMEARS

Question 1

Romanowsky stains

Answer 1

-Basic stain (methylene blue ) +acidic stain (eosin)
-Methylene blue on oxidatn gives azure (coloured compound)
-azure combines with eosin (C-)
-azure, gives different shades of stains in the smear
RBC s ,pink
Granular of easnophils, red orange
Granules of basophils, bluish black
Granules of neutrophils, lilac

Question 2

Basophilic and acidophilic stains

Answer 2

*structures taking acidic dye ,acidophilic/ eosinophilic
Eg cytoplasm

*structures taking basic dyes
Eg,nuclei and structures