Hematocytometer

Question 1

Hematocytometer/cell counting chamber

Answer 1

Counting chamber
Used for manual counting blood cells

Question 2

Hematocytometer invented by

Answer 2

Louis Charles malassez

Question 3

Hematocytometer consists of

Answer 3

Thick glass microscope slide
Rectangular identation create precision volume chamber

Question 4

Principle of hematocytometer

Answer 4

No of cell counter=sum of cells counted across square in one chamber

Question 5

Different types of counting chamber

Answer 5

1,Thoma cell counting
2,malassez
3,Improved new vauer
4,burger
5,fuch rasanthal

Question 6

Thoma cellcounting

Answer 6

Contain a central square with 1 mm with 10x objective
*for ruling leukocytes and hemoglobin

Question 7

Chamber Used to count nematodes from cerebrospinal fluid

Answer 7

Malassez

Question 8

Principle of malassez

Answer 8

No of particles per unit volume
For particles within the particles (leucocytes, erythrocytes)

Question 9

Improved newbauer

Answer 9

3mm ×3mm area
9 squares with 1 mm each
4 corner square to Determine WBC count which 8s subdivided into16
*to determine RBC ,4 corner squares and central squares(1/5) of 25 subdivided portion of the central square is used

Question 10

Covers slip for counting chamber

Answer 10

Can count under low power and high power but not for oil immersion
3 thickness and 2 sizes
Thickness-0.3,0.4(common),0.5
Size-16×22 (single ruled) and 22×23(double ruled)

Question 11

Changing counting chamber

Answer 11

Raised shoulder of chamber and coverslip should fit properly -to ensuredepth of the chamber is uniform
*the diluted blood should does not overflow to the H shaped trough, so are must be needed
*drop added to edge of cover blast and drawn by capillary action
*for counting platelets, simple moist chamber by placing a cotton/wet pad on petridish can be used
*before counting, allow cells to settle in the moist chamber