ESR

Question 1

ESR

Answer 1

State at which erythrocyte sediment when anticoagulant blood is allowed to stand in a vertical column

Question 2

Methods of ESR

Answer 2

Westergrens method, modified Westergrens method
Disposable:wintrobes
Zeta sedimentation

Question 3

Principle of estimate of ESR

Answer 3

-anticoagulated venous bloody in vertical tube undisturbed
-erythrocyte falls towards the bottom
-2 layers, upper plasma(in multi meter for 1 hr)and lower red cell layer
-process erythrocyte sedimentation
-rate ESR

Question 4

Requirements for westegrins method

Answer 4

-Pipettes
Both side open
30 cm in length
Bore size-2.5 mm
Caliberated in mm for 0 to 200
Capacity-1ml

-westegrins stand
-anticoagulant
3.8%na citrate soln
2ml of whole blood in o.5ml of 3.8%na citrate of blood to anticoagulant ratio 4:1

Question 5

Westegrens methods forESR

Answer 5

Westegrins pipette filled upto 0 mark
Placed vertically in rack
At room temp
Without vibration or direct sunlight

Question 6

Normal value of ESR using westegrins

Answer 6

Adult males,children- 0 to 10 mm/1 hr
Adult females-0 to 20mm /1 hr

Question 7

Significant of ESR

Answer 7

-prognostic test than diagnostic test
-helps in monitoring activity &nonspecific marker in underlying inflammation

Question 8

Cause of ESR^

Answer 8

Multiple myeloma
Mucroglobulineoma
Osteomylitis
Rheumatoid fever
Tuberculosis
Rheumatoid arthritis
SLE
neoplasia

Question 9

Cause of decreased ESR

Answer 9

Polycythemia vera
Sickle cell disease
Hypofibrinodenemia

Question 10

Modified westegrins, method

Answer 10

Blood anticoagulated with EDTA,not with citrate/ oxalates
Advantage
Blood collected from same tube from which coagulation studies are performed

Question 11

Disposable method

Answer 11

More convenient
Eliminate risky method of filing blood through pipette
ESR only fills blood upto 0 mark which sucks!

Question 12

Winetrobes method

Answer 12

Wintrobes tube filled upto 0 mark(C- EDA/double oxalate anticoagulated blood)
-blood tube filled in vertical position
-after 1hr ,read upper plasma in mm/hr

Question 13

Advantage of wintrobe method

Answer 13

Small amount of blood and simple
Some tube may used for ESR &PCV
Buffy coat smear useful in leukopemia, subleukemic leukemia &LE cell test

Question 14

Automated method for ESR estimation

Answer 14

1,vesmatic
2,ESR STAT PLUS
3,SEDIMAT
4,Zeta sedimentation

Question 15

VES MATIC

Answer 15

To measure 20 blood sample
Result-comparable to westegrin method
Available in approx 22 min

Principle
-blood in special cuvette
-sample mixed by instruments ,allowed to stand at 18o stand from vertical
-opto electrical sensor measure ESR
-data ,elaborated &printed

Question 16

ESR STAT PLUS

Answer 16

Centrifugation based
Result in 5 min

Question 17

SEDIMAT

Answer 17

-In sedimat automated ESR reader, filled sediplast westegrins pipet is placed
Accelerate sedimentation under controlled conditions
-after 15 min on LCD display, reader displays the result
-result stored in memory and can be printed

Question 18

Zeta sedimentation ratio

Answer 18

-In small bore capillary tube filled with blood
-rotate for 3 to 4 min in Zetafuge (special centrifuge)
-centrifuge alternatively and disperse RBC under standardized centrifuged force
-tube read in zetacrit(special reader)to obtain value

Question 19

Advan of ZSR

Answer 19

Rapid
Corrects for anemia
Require small blood sample
Desirable for pediatric patients