Anticoagulants

Question 1

After how many times the blood clots

Answer 1

10 minutes

Question 2

Anticoagulants

Answer 2

Chemicals that prevent blood coagulation

Question 3

Most anticoagulants can be coagulation by removing ca++ except.comment

Answer 3

Heparin destroys thrombin ,thromboplastin

Question 4

Name some anticoagulants

Answer 4

EDTA
double oxalate
Na citrate
Heparin
CPD (citrate phosphate dextrose)
ACD(acid citrate dextrose) and SPD(na phosphate dextrose)in blood bank

Question 5

Most of the anticoagulant coagulates blood by which mechanism

Answer 5

Chelating ca++(removing ca++)

Question 6

EDTA used in routine morphology except coagulation studies .why?

Answer 6

Cz it maintains blood cell morphology

Question 7

EDTA

Answer 7

1.2 mg/ml of blood is used
RBC shrinks and blood cells are degenerated at high conc
Demerits:aggregation of WBC and platelet adhesion

Question 8

The form in which double oxalate is used.Reason?

Answer 8

Mix of ammonium and potassium oxalate
K ox-shrinks RBC and nh3 ox-swells.
0.8 g of k ox and 1.2g of nh3 ox in 100 ml of distilled h20(o.2 ml of 5ml blood )is used

Question 9

Some Anticoagulants used for ESR estimation

Answer 9

Double oxalate and trisodium citrate

Question 10

Trisodium citrate quantity to be taken

Answer 10

3.8 for ESR
3.1for coagulation studies

Question 11

How trisodium citrate is used

Answer 11

9 vol of blood:1 vol of anticoagulant ratio
Used in liquid form

Question 12

The way heparin acting instead of chelating ca++

Answer 12

By inhibiting the action of thrombin

Question 13

Heparin used for osmotic fragility test.why?

Answer 13

It does not alter pH of media and size of RBC

Question 14

Uses of heparin

Answer 14

Blood gas and electrolyte analysis
Osmotic fragility test
Not used in total count,cz it clumps the WBCs
Not used in peripheralsmear ,cz it gives bluish bg

Question 15

Anticoagulant used in westeergren method

Answer 15

EDTA